Bcmb 406b - Study guides, Class notes & Summaries

what variables are considered in primer design length, sequence, %GC, Tm, Ta, 3'end stability, 5' end stability, secondary structures why is the formation of secondary structures in primers especially undesirable reduces primer available to use to make product Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More standard denaturation temp and time (initial and denaturation) 94C 1-5min initial 20-45 sec...

BCMB 406B Test 1Latest Update ...

The success of a PCR reaction is mostly dependent on what? well designed primers Primer design can also be applied to what? - probes in Southern and Northern blots - DNA sequencing - microarray design Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More What else can affect the success of a PCR reaction? - Quality of the template DNA - cycling conditions - Specificity and stability of the PCR primers ...

Glycoside hydrolases Hydrolyze the glycosidic bond between 2 or more carbohydrates or carbohydrate-protein bonds Carbohydrate Binding Modules - Amino acid sequence within a carbohydrate-active enzyme with a discreet fold that has carbohydrate binding activity - Maintains the interaction of the enzyme with its substrate Previous Play Next Rewind 10 seconds Move forward 10 seconds Unmute 0:00 / 0:15 Full screen Brainpower Read More Vector NTI Advance - Allows you to...

BCMB 406B - Lab 1 Exam Questions With Correct Answers The success of a PCR reaction is mostly dependent on what? - answerwell designed primers Primer design can also be applied to what? - answer- probes in Southern and Northern blots - DNA sequencing - microarray design What else can affect the success of a PCR reaction? - answer- Quality of the template DNA - cycling conditions - Specificity and stability of the PCR primers What are key criteria in primer design? - answer- Primer length...

Reasons for sequence errors within PCR products - Answer Low fidelity of DNA polymerase, excess Mg2+ concentration, unbalanced dNTP concentrations, high number of cycles, UV-damaged DNA, or sequencing error Reasons for sequence error at termini of PCR products - Answer Problematic primer design, low primer quality, contaminating nucleases, UV-damaged DNA, or sequencing error