Steps of pcr - Study guides, Class notes & Summaries
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WGU c785 2023 with all the correct answers(Actual test 100% verified)
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WGU c785 2023 with all the correct 
answers(Actual test 100% verified) 
polymerase chain reaction (PCR) - CORRECT ANSWER process to copy DNA 
What is needed to complete PCR - CORRECT ANSWER Template DNA, 
Nucleotides (dNTPS), DNA Polymerase, and DNA 
Steps of PCR - CORRECT ANSWER denaturation, annealing, elongation 
denaturation - CORRE
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WGU Biochemistry OA (Latest 2022/2023) Verified Answers
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WGU Biochemistry OA (Latest 2022/2023) Verified Answers 
Bohr Effect chart (low pH) 
C- High CO2 
H- High H+ 
A- Acidic 
R-Release of O2(rt. Shift) 
T- tense state (in tissues) 
 
 
Primary structure 
Sequence of amino acids that make up a protein 
 
 
Secondary Structure 
Sequence of amino acids fold. 
 
 
Tertiary Structure 
-more folding in a 3D shape. 
-R groups (side chains) 
 
 
Quaternary Structure 
Protein has more than 1 polypeptide chain. 
 
 
Chemical reaction 
-absorb and release en...
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BCH5413 TEST 2 QUESTIONS AND ANSWERS ALL REVISED AND UPDATED
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BCH5413 TEST 2 QUESTIONS AND ANSWERS ALL REVISED AND UPDATED 
Methylation: a primitive immune system for bacteria 
- hds-R - Answer-- plasmid transforms into cells are protected (restriction enzyme not expressed) 
 
Methylation: a primitive immune system for bacteria 
- mcrA/mcrB/mrr - Answer-degrades foreign DNA that is not properly methylated 
- can occur to DNA obtained from mouse/human cells containing CpG methylated DNA (can interfere with cloning of mammalian genomic DNA) 
 
pCR 2.1 - TOP...
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BCH 5413 EXAM ONE QUESTIONS WITH VERIFIED ANSWERS
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BCH 5413 EXAM ONE QUESTIONS WITH VERIFIED ANSWERS 
If you want to identify the protein PTEN on a Western blot, what is secondary antibody? - Answer-Fab can bind to primary antibody and Fc portion can bind to a HRP antibody so when bound to TMB will light up 
 
Which of the following increases the stringency of Southern Blot? Why? - Answer-Increased hybridization, more Interactions between target and probe 
 
PCR: primer - Answer-Short ssDNA sequence used in PCR, pair of primers used to hybridize...
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WGU Biochemistry Final Review 2024 With Complete Solutions Latest Guide A+.
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WGU Biochemistry Final Review 2024 With Complete Solutions Latest Guide A+. 
1. The polymerase chain reaction is a tool used to study protein structure. 
True 
False - ANSWER-False 
Feedback 
PCR is a tool used to amplify a specific segment of DNA. 
What color is the primer in the following diagram? 
Red 
Purple 
Blue - ANSWER-Red 
Feedback 
Recall from the section on DNA replication that DNA polymerase needs a primer to 
begin DNA synthesis. This requirement means the primers will direct the DN...
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WGU C785 BioChem OA Review | 100% Correct Answers | Verified | Latest 2024 Version
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Males and Females are equally affected - Autosomal 
The process of copying DNA in the lab. - Polymerase Chain Reaction (PCR) 
Template DNA, Nucleotides (dNTPS), DNA Polymerase, and DNA Primers. - What is needed for 
Polymerase Chain Reaction? 
Denaturing, Annealing, Elongating - What are the three steps of a Polymerase Chain Reaction (PCR)? 
Denaturing (DNA is heated up to separate it) - What is the first step of a Polymerase Chain Reaction? 
Cooling the Polymerase Chain Reaction. Primers stick ...
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WGU c785 Final Exam | 100% Correct Answers | Verified | Latest 2024 Version
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Hemophilia Pedigree - Father has hemophilia, mother does not. What is the outcome for their kids? - 
His daughters would be carriers. This is x-link recessive. 
Autosomal: 
Dominant: - Autosomal: males and females equally affected. 
Dominant: non-carrier parents 
polymerase chain reaction (PCR) - The process of copying DNA in the lab. Uses Template DNA, 
Nucleotides (dNTPS), DNA Polymerase, and DNA primers. 
3 Steps of PCR - 1. Denaturation: DNA is heated to 95C to separate it. 
2. Annealing: re...
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ASU BIO 340- Exam 3 ( Recitations 8-11)
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During PCR, two DNA strands are separated by breaking hydrogen bonds during the denaturation step 
T or F 
True 
 
 
 
Which of the following is true about PCR? 
a. only one primer is needed for PCR to proceed 
b. the purpose of PCR is to amplify a region of DNA 
c. PCR requires a hot start of 60 c 
d. PCR melts the covalent bonds between DNA strands 
b. the purpose of PCR is to amplify a region of DNA 
 
 
 
 
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WSU Bio 102 Final Exam Questions and Answers Already Passed
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WSU Bio 102 Final Exam Questions and 
 
Answers Already Passed 
 
How does BPA cause cancer? it disrupts meiosis and mitosis causing breast and prostate 
cancer. 
 
3 and 7 plastics may contain BPA 
 
What is a genetic fingerprint? how do you make one? -Genetic Fingerprint: DNA uses the 
differences in base sequences to identify individuals. 
-DNA fingerprinting involves 3 tools: 
1. PCR to get large amounts of DNA 
2. Restriction digest with blunt ends to fragment the DNA with precision 
3. Gel...
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WGU Biochemistry Final Review 2024 With Complete Solutions Latest Guide A+.
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WGU Biochemistry Final Review 2024 With Complete Solutions Latest Guide A+. 
1. The polymerase chain reaction is a tool used to study protein structure. 
True 
False - ANSWER-False 
Feedback 
PCR is a tool used to amplify a specific segment of DNA. 
What color is the primer in the following diagram? 
Red 
Purple 
Blue - ANSWER-Red 
Feedback 
Recall from the section on DNA replication that DNA polymerase needs a primer to 
begin DNA synthesis. This requirement means the primers will direct the DN...
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