Sds page 3 - Study guides, Class notes & Summaries

how much BSA is needed to prepare 50 mL of 5 mg/ml of BSA (w/v)? - Answer-(50 mL)(5 mg,mL) = 250 mg a technician is assigned the task of inserting a gene, LanA, into a plasmid to ultimately be expressed as a therapeutic protein in E coli. label the following steps (1-4) in the correct order the technician must perform them to accomplish her task. - Answer-1) design primers to amplify the lanA sequence using polymerase chain reaction (PCR) and perform the PCR reaction 2) digest and provide ...

Lead Duties: what is your number one priority and name the two categories - Answer- number one priority is to be a role model for the instructors on your team. Two categories are administrative and clinical duties Lead duties: Administrative duties (name and describe all 5) - Answer- 1) upkeep all program materials including the log box and all documents contained in the log box: -service verification forms - program plans (program title, SDs, responses and targets all current and dated) ...

pKa (chapter 3) - measure of the tendency of a group to give up a proton (acidity); tendency decreases tenfold as pKa increases by one unit thioester bond (1) - compounds with functional group C-S-CoA-C (eg, in acetyl-CoA) zwitterion (3) - dipolar ion with both positive and negative groups but overall neutral charge; can act as either an acid or base Isoelectric point (pI) (chapter 3) - the characteristic pH at which the net electric charge is zero SDS - Sodium Dodecyl Sulfate (chap ...

ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS Henderson-Hasselbach Equation - answerpH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - answerUsed in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - answerChanges soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - answerSeparates sample b...

QBM Exam 3 Questions With Complete Answers Graded A+ Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? miniprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? Cesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? Miniprep. Which of the following wavelengths is incorrectly matched? 240 nm - RNA concentration. The absorbance of a DNA sample at A260 = 1...

Medical Biochemistry, UCF questions and answers latest top score. Alanine - correct answers.Ala, A Arginine - correct answers.Arg, R Asparagine - correct answers.Asn, N Aspartic Acid - correct answers.Asp, D Experimental techniques: PCR - correct answers.-can amplify small amounts of DNA Experimental techniques: Western blot - correct answers.-measures protein levels in a sample using antibodies Experimental techniques: Southern blot - correct answers.-de...

ACS BIOCHEMISTRY EXAM QUESTIONS AND ANSWERS Henderson-Hasselbach Equation - answerpH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - answerUsed in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - answerChanges soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - answerSeparates sample b...

ACEDS/ACTUAL EXAM LATEST VERSION WITH VERIFIED QUESTIONS AND ANSWERS GRADED A+ Henderson-Hasselbach Equation - ANS--pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANS--Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Collagen - ANS--Repeating sequence of Gly-X-Pro. 3 stranded "coiled coil". Contains gly core. Myoglobin 4° Structure - ANS--Symmetric homodimer, Salting Out (Purification) - ANS--Changes...

QBM Exam 3 Questions With Complete Answers Graded A+ Which of the following DNA purification techniques CANNOT be used for genomic DNA purification? miniprep Which DNA purification technique involves purifying the sample using an ultracentrifuge? Cesium chloride gradient What technique is used to increase plasmid copy number and purify plasmids? Miniprep. Which of the following wavelengths is incorrectly matched? 240 nm - RNA concentration. The absorbance of a DNA sample...

What is biotin and digoxigenin most commonly used for in the lab? Why are they safer? Probe labeling (aka tagging nucleic acids so that they can be visualized). They are safer because they are nonradioactive. Name the three basic methods used to label a DNA probe: 1. End Labeling 2. Nick Translation 3. Random priming What is SDS? Name 3 of its applications in the lab. Sodium Dodecyl Sulfate. A detergent. 1. Denatures proteins. 2. Used in SDS-PAGE to denature proteins so they separat...