Sds page - Study guides, Class notes & Summaries

SDS-Page abbreviated for Correct Answer-Sodium Dodecyl sulfate Polyacylamide Gel Electrophoretic SDS in the presence of protein and heat Correct Answer-wraps around the protein polypeptide backbone and coat it with negative charges to the protein in order to make sure the protein have the same negative charge/mass ion. The shape of the protein is now linearlized and denatured polypeptide How and where does SDS bind to protein Correct Answer-How? by hydrophobic interaction between dodceyl g...

BCHM 270 EXAM QUESTIONS WITH CORRECT ANSWERS A physician would like to evaluate the elevated amounts of a gene being expressed in two different types of tumor cells in order to develop a personalized medicine. Which of the following techniques would be the most appropriate for this purpose? A. Western Blot B. Southern Blot C. Northern Blot D. Eastern Blot E. enzymatic assay - Answer-C A restriction enzyme can be used for which techniques? A. Southern blots and cloning. B. western blo...

AAMC FL 5 Questions and Answers 2023 Question 6 Thought process: - Didn't know the structure of flavin, therefore needed to narrow down answer from answer choices: - Knew A was a pyrimidine, cross that out - Had not seen structure of B before - Knew C was ubiquinone (still chose just because ubiquinone was mentioned in the passage (stupid)) - Didn't try and analyze structure of D at the time Correct thought process: - Cross out A bc pyrimidine - Never seen B befor...

Henderson-Hasselbach Equation - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - Separates sample based on size with smaller molecules eluting later. Ion-Exchange...

Western blotting a. Proteins from SDS-PAGE are transferred to a nitrocellulose membrane using electrical current a. membrane is blocked so antibody will not bind nonspecifically b. Primary antibody is added and incubated with the membrane c. To detect antibodies that have bound to protein a secondary anti-immunoglobulin antibody coupled to a reporter a. Excess secondary antibody is washed off the membrane b. Substrate is added that precipitates upon reaction c. Reveals visible band where...

ACS BIOCHEMISTRY EXAM QUESTION WITH COMPLETE SOLUTIONS LATEST 2024. Henderson-Hasselbach Equation - CORRECT ANSWER - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - CORRECT ANSWER - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the Nterminus. Salting Out (Purification) - CORRECT ANSWER - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution....

ACS BIOCHEMISTRY EXAM QUESTION WITH COMPLETE SOLUTIONS LATEST 2024. Henderson-Hasselbach Equation - CORRECT ANSWER - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - CORRECT ANSWER - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the Nterminus. Salting Out (Purification) - CORRECT ANSWER - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. ...

BACE Review Questions and Answers 2024 Structural component of cell membrane, Ex: Phospholipid Lipid A chemical bond that involves the sharing of electron pairs between atoms. What is the most likely reason there is an unexpected band in a PCR reaction? Using too low temp. What should be included on the gel to enable you to determine actual sizes of the DNA fragments? Molecular weight marker and DNA Ladder What is MRNA? What the order of amino acids on a polypept...

ACS BIOCHEMISTRY Solved Exam |100% Pass| Henderson-Hasselbach Equation *Ans* pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis *Ans* Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) *Ans* Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography *Ans* Separates sample based on ...

Rank the following biological molecules in order of how readily they diffuse across the plasma membrane from the most diffusible to the least diffusible. I. CO2 II. Cl III. Sucrose IV. Glycerol A. I, III, IV, II B. II, IV, III, I C. III,II, IV, I D. I, IV, III, II E. II, I, III, IV correct answersAnswer: I, IV, III, II Explanation: carbon dioxide diffuses the easiest, shown by gas exchange in capillaries; glycerol is lipid soluble; sucrose is too large to cross the membran...