Histotechnology final Study guides, Class notes & Summaries

Histotechnology test 2What is the consequence to improper clearing? - correct answers "Moth eaten" due to water still being in the tissues- improper dehydration Sections will crumble and fall out of block Shrink the centre of the tissue Why would a tissue shrink? - correct answers Wax is too hot What causes microscopic chatter? - correct answers Over dehydration seen most prominately at the ends of tissues What is the melting point do most paraffins? - correct answers 55-58 degr...

Place in a separate container CORRECT ANSWER-Match each listed item with the appropriate disposal method: Used silver nitrate Simply pour down the sink CORRECT ANSWER-Match each listed item with the appropriate disposal method: 1% alcohol Dilute first, pour down the sink CORRECT ANSWER-Match each listed item with the appropriate disposal method: Concentrated HCL Place in a biohazard bag CORRECT ANSWER-Match each listed item with the appropriate disposal method: Blood-stained...

Histotechnology I FINAL EXAM PREP with complete solutions

What are the two common staining methods for nucleic acid in histology? CORRECT ANSWER-1. Feulgen Reaction 2. Methyl Green-Pyronin Method (DNA/RNA) Feulgen Reaction tests for what? CORRECT ANSWER-DNA What does the Schiff reagent bind to in the Feulgen Reaction? CORRECT ANSWER-Aldehyde group created by the hydrolysis of DNA Why can't we use the Bouin fixative on tissues being tested with Feulgen? CORRECT ANSWER-Bouin hydrolyzes tissues In Feulgen reactions, what happens when the hyd...

Explain the steps involved in tissue processing - ANSWER-1. Surgery 2. Fixation 3. Grossing 4. Fixation 5. Processing 6. Embedding 7. Staining 8. Cover-slipping Discuss the process of fixation - ANSWER-Stabilization of proteins to prevent autolysis and putrefaction. -Maintain proper relationship between cells and extracellular material (keep as "life-life" as possible) -15-20 times volume fixative of tissue -10% NBF most common fixative Describe the goal of tissue proces...

Histotechnology I--Lecture Final Exam Review 2023...

Histotechnology I--Lecture Final Exam Review 2023...