Dna polymerases - Study guides, Class notes & Summaries

Which of the following build(s) new strands of DNA? - ANSWER DNA polymerases Which statement about DNA replication is CORRECT? - ANSWER The leading strand is built continuously, and the lagging strand is built in pieces. The molecule that seals the gaps between the pieces of DNA in the lagging strand is - ANSWER DNA ligase. Which of the following events occurs during transcription? - ANSWER A molecule of RNA is formed based on the sequence of nucleotides in DNA. mRNA? - ANSWER mRNA m...

The sequence of one strand of DNA is shown below. Which of the following is the sequence of its complementary strand? 3′ ATTTGGCC 5′ 5' TAAACCGG 3' DNA polymerases are enzymes that build DNA. They catalyze ____ reactions leading to the formation of ____ bonds. condensation; phosphodiester A bacterium is infected with an experimentally constructed bacteriophage composed of the T2 phage protein coat and T4 phage DNA. The new phages produced would have -------. T4 protein and T4 DNA Which ...

BCH 5413 EXAM ONE QUESTIONS WITH VERIFIED ANSWERS If you want to identify the protein PTEN on a Western blot, what is secondary antibody? - Answer-Fab can bind to primary antibody and Fc portion can bind to a HRP antibody so when bound to TMB will light up Which of the following increases the stringency of Southern Blot? Why? - Answer-Increased hybridization, more Interactions between target and probe PCR: primer - Answer-Short ssDNA sequence used in PCR, pair of primers used to hybridize...

Nucleic Acid grows by attachment of 5' phosphate group of the incoming nucleotide to the 3'hydroxyl group of the last nucleotide double stranded DNA most energetically favorable state for DNA Purines double-ring structure Adenine (A) + Guanine (G) Pyrimidine single-ring structure Uracil (U)+ Cytosine Messenger RNA (mRNA) -template for all protein synthesis -encode all information necessary to produce proteins Heterogeneous nuclear RNA primary product o...

Nucleic Acid grows by attachment of 5' phosphate group of the incoming nucleotide to the 3'hydroxyl group of the last nucleotide double stranded DNA most energetically favorable state for DNA Purines double-ring structure Adenine (A) + Guanine (G) Pyrimidine single-ring structure Uracil (U)+ Cytosine Messenger RNA (mRNA) -template for all protein synthesis -encode all information necessary to produce proteins Heterogeneous nuclear RNA primary product o...

MMSC490 Exam 2 Questions with 100% Correct During E. coli DNA replication, all newly synthesized or nascent DNA fragments must have one of the following characteristics a. a 5' hydroxyl and 3' triphosphate b. a 5' triphosphate and 3' monophosphate c. 5' monophosphate and 3' hydroxyl d. 5' triphosphate and 3' hydroxyl - Answer ️️ -d. 5' triphosphate and 3' hydroxyl Among the 3 DNA polymerases in E. coli, DNA polymerases I and III differ in a number of properties that make DN...

The structure of a strand of DNA. - it is a double helix, like a twisted ladder. the ladder rings are nucleotide subunits and they have a covalent bond to the sugar phosphate backbone (made of deoxyribose and a phosphate group) What are the nucleotide subunits? - adenine, guanine, cytosine, and thymine How are the subunits held together? - through a hydrogen bond What are the nucleotide subunits compliments? - Adenine and thymine cytosine and guanine How does DNA replicate? - the parent s...

Similarities between eukaryotic and prokaryotic cells 1) Semi-conservative 2) Bidirectional Prokaryotic cells 1) Circular DNA 2) Single origin of replication Eukaryotic cells 1) Linear DNA 2) Multiple origins of replication New strands are always synthesized from: 5' --> 3' end of each strand Template strand is read: 3' --> 5' Chain extension occurs: by adding a dNTP to free 3' end of growing polynucleotide chain DNA polymerase DNA s...

TEST BANK for Fundamentals of Molecular Virology 2nd Edition by Acheson. Package Title: Testbank Course Title: Acheson 2nd edition Chapter Number: 01 Question type: Multiple Choice 1) Which of the following terms describes the protein shell that surrounds the viral genome? a) capsid b) envelope c) matrix d) virion e) capsomere Answer: a 2) Which of the following would not be a nucleic acid form found in a viral genome? a) dsDNA b) ssDNA c) dsRNA d) ssRNA e) an RNA:DN...

Topic 1 Introduction to Molecular Cloning 1. The goal of this integrated practical course is to purify a protein. In general, what is the value of protein purification to medical research? What information can a purified protein provide and what techniques can be developed/employed once you have a purified protein?  In vitro studies - Small molecule interactions, protein binding partners etc  Producing antibodies  Determination of structure Purification of the protein is a valua...