Dna plasmid - Study guides, Class notes & Summaries

BCH5413 TEST 2 QUESTIONS AND ANSWERS ALL REVISED AND UPDATED Methylation: a primitive immune system for bacteria - hds-R - Answer-- plasmid transforms into cells are protected (restriction enzyme not expressed) Methylation: a primitive immune system for bacteria - mcrA/mcrB/mrr - Answer-degrades foreign DNA that is not properly methylated - can occur to DNA obtained from mouse/human cells containing CpG methylated DNA (can interfere with cloning of mammalian genomic DNA) pCR 2.1 - TOP...

Microbiology Exam 100 Questions & Answers 2023/2024 UpdateMicrobiology,Oral Physiology,Oral Diagnosis, Pathophysiology,Phamacology, Class Anesthesiology & General Physiology Exam Questions & Answers 2023/2024 Update 1. Any deviation from health where tissues and organs are damaged and disrupted is termed as: A. Pathology B. Disease C. Infection D. Transient microbiota E. Commensalism 2. The human body provides a favorable habitat for microorganisms. Which of the following ...

BIOE 206 Exam 1; Questions and Answers 100% Correct Plasmid DNA purification steps Correct answer-1. grow the bacteria with the plasmid 2. centrifuge 3. remove supernatant 4. resuspend pellet in lysis buffer (detergents and high salt) 5. neutralize the lysis buffer 6. spin down to remove debris and genomic DNA 7. put the suspension on a spin column 8. plasmid DNA will adhere to spin column and everything else will be washed away with high salt buffers 9. add low salt buffer to remo...

loop of Henle correct answers where Na+ is reabsorbed from the filtrate moving through the nephron This is ultimately the mechanism whereby the kidneys concentrate urine - section of the nephron tubule that conserves water and minimizes the volume of urine action potentials result in correct answers an increased permeability of the plasma membrane to sodium - Na+ in causes depolarization hyperglycemia promotes cellular dehydration because correct answers glucose molecules raise the osmo...

CMMB 343 Exam Study Guide with 100% Correct Answers | Verified | Latest 2024 Version How does phi6 penetrate the host cell? - because it is an enveloped phage, it uses the entry and uncoating model that relies upon spike-protein mediated endocytosis - once in the vesicles, it releases the viral particles and proteases digest the capsid coat, lysing the vesicle, and releasing the phage genome into the cytoplasm Which protein is the plug at the end of the tail tube in T4, preventing DNA from...

IB Biology semester 1 Latest Version Graded A+ Cells Smallest functional unit of an organism What are the principles of cell theory? 1. All living things are made of cells 2. Cells are smallest unit of life 3. Cells come from pre-existing cells What are the properties of life? Metabolism Reproduction Growth Response Excretion Homeostasis Nutrition Prokaryotic cells Single cell which has neither a distinct nucleus or organelles can be an organism (e.g. bacteria) Flage...

WSU Bio 102 Final Exam Questions and Answers Already Passed How does BPA cause cancer? it disrupts meiosis and mitosis causing breast and prostate cancer. 3 and 7 plastics may contain BPA What is a genetic fingerprint? how do you make one? -Genetic Fingerprint: DNA uses the differences in base sequences to identify individuals. -DNA fingerprinting involves 3 tools: 1. PCR to get large amounts of DNA 2. Restriction digest with blunt ends to fragment the DNA with precision 3. Gel...

What is antibiotic selection? Correct Answer-Survival of a population of antibiotic resistant cells within a larger population of antibiotic sensitive cells. Where can antibiotic selection occur? Correct Answer-Anywhere where there are antibiotics and bacteria present. The best-surviving bacteria will be selected for. Name a common use for antibiotic selection in microbiological research. Correct Answer-To identify and isolate cells that have piece of DNA of interest such as a particular p...

BIOE 206 Exam 1 UPDATED ACTUAL Questions and CORRECT Answers Plasmid DNA purification steps - CORRECT ANSWER- 1. grow the bacteria with the plasmid 2. centrifuge 3. remove supernatant 4. resuspend pellet in lysis buffer (detergents and high salt) 5. neutralize the lysis buffer 6. spin down to remove debris and genomic DNA 7. put the suspension on a spin column 8. plasmid DNA will adhere to spin column and everything else will be washed away with high salt buffers 9. add low salt ...

BIO 205 Exam Questions With 100% Correct Answers Nucleoid is a region/structure found only in ____ cells. a) prokaryotic b) eukaryotic c) bacterial d) Both A and C Prokaryotic/bacterial A plasmid is double-stranded DNA. True or false? True A plasmid-gene or a chromosomal-gene (piece of DNA with a specific product) will always produce a________. a) carbohydrate b) ATP c) Protein d) Lipid Protein DNA polymerase is a(n) a) lipid b) polysaccharide c) enzyme d) nucelic ...