Crispr cas9 - Study guides, Class notes & Summaries

CMMB 403 final exam what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - Answer ️️ -- nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR - Answer ️️ -endonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 - Answer ️️ -1. non-homologous end joining (NHEJ) - nat...

CMMB 403 Final Exam with complete solution what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - answer- nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR - answerendonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 - answer1. non-homologous end joining (NHEJ) - natura...

CMMB 403 final examwhat does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - correct answer - nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR - correct answer endonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 - correct answer 1. non-homologous end joining (NHEJ) - n...

How does CRISPR work naturally? Correct Answer Clustered Regularly Interspaced Short Palindromic Repeats - bacterial defense mechanism - bacteria have short palindromic repeats separated by spacer regions which are segments of DNA from past viral exposure - when attacked again by same virus, the spacer region is used as a template and the guide RNA (tracrRNA) helps recognize the viral DNA and degrade it using Cas9 nuclease at the specific site - need PAM (helps distinguish between self and n...

Deze samenvatting bevat alle onderwerpen behandeld tijdens minor 13 (molecular cloning, genetically modified organisms, CRISPR/Cas9, vaccines, therapeutic antibodies, genetic therapy and its tool box, molecular diagnostics, gene therapy, stem cells & tissue engineering en research involving humans).

Module 6: Genetic Engineering Exam Questions With Verified Answers. How are ds breaks repaired - answernon-homologous end joining (NHEJ) homologous directed recombination (HDR) non-homologous end joining (NHEJ) definition - answerligated 2 broken ends together (not template repaired) typically results in insertions or deletion homologous directed recombination (HDR) definition - answertemplate dependent pathway for DSB repair can insert donor molecule at target types of application of ...

CRISPR-Cas9 Complete Study Guide Exam 2024. What is CRISPR-Cas9 used for? - CORRECT ANSWER Recently developed method for making precise and targeted changes in the genome of a living cell What are the advantages of using CRISPR-Cas9? - CORRECT ANSWER Fast, efficient, works in many different species, not limited by bg strain, doesn't produce chimeras, no molecular scar left behind Because CRISPR-Cas9 is so use friendly what has it done for research? - CORRECT ANSWER Open all kinds of ...

The two polynucleotide chains in DNA are held together primarily by H bonds between A and U as well as between G and T? a. True b. False correct answers B (DNA does not have Uracil, it has Cytosine) If there is 12% of A in a DNA molecule, then the % of G must be? a. 12% b. 30% c. 368 d. 76% e. none of these are correct correct answers E (A=T and C=G, A+G/T+C=1) The catalytic molecule responsible for making a peptide bond during translation (protein synthesis) in cells is? ...

CMMB 403 final Exam New Year 2024| Questions and Answers| Certified Quality Graded A+ what does CRISPR use in order to generate changes in genome? is CRISPR forward or reverse genetics? - nuclease - 20 nucleotide RNA sequence to target specific sites in the genome - reverse genetics what is the role of Cas9 in CRISPR endonuclease that catalyzes double stranded breaks what are the two repair paths cells can use to repair double stranded breaks from CRISPR-Cas9 1. non-homologous end jo...

CRISPR Cas-9 Questions With Correct Actual Answers 2024. How many nucleotides long is the guiding region of the sgRNA? - CORRECT ANSWER 20 nucleotides Does the sgRNA bind to the PAM? - CORRECT ANSWER Yes, there is a spacer on the sgRNA that is within the Cas9 protein that binds to the protospacer sequence, opening up the double helix Where does Cas9 cut the target DNA relative to the protospacer sequence? - CORRECT ANSWER It cuts the DNA 3 nucleotides upstream of the PAM Describe i...