CHEM MISC

Quiz 2 Biochemistry Lab Chem 378 Name:____________________ 1. Below are restriction enzyme maps of two plasmids. Draw or described in numbers the expected bands of digestion in 1% agarose gel electrophoresis, relative to the 2 bands identified for you as control. 2. Explain the different steps that are involved in a PCR experiment (include all the reagents used in the PCR mixture). 5.5 kb BamHI 3730 PstI 2650 BamHI 3050 PstI 1720 PstI BamH1 PstI BamHI pCAG-EYFP 4000 bp 3000 ...