Expression cloning - Guides d'étude, Notes de cours & Résumés

EPPN 154 Exam 3 Questions & Answers 2024/2025 Expressing foreign genes in bacteria - ANSWERSTranscription and translation to produce coded proteins Obstacles in expressing foreign genes in bacteria - ANSWERS1. Genes placed under control of a bacterial promoter 2. Introns must be removed 3. Codons might need editing 4. Many eukaryotic proteins require host modification after translation Expression Vectors allow for control of what? - ANSWERSexpression of the cloned genes How mus...

What is the purpose of having rrnBT4 in an expression vector? - correct answerEnhances the stability of the GOI transcript Why must reverse transcriptase be used to create a eukaryotic expression library? - correct answerThe resulting cDNAs do not contain non-protein coding introns Which type of ligation-independent cloning technique takes advantage of the recombination between lambda phage attB and attP sequences in the vectors and in the DNA fragment to be inserted? - correct answerGatew...

BIO 1A03 Midterm 2 Questions and Answers Graded A Prokaryotic growth and cell division -nutrient-rich environment -favorable temperatures Housekeeping genes -genes required at all times for normal function -all ways transcribed and translated -allow constant maintance for general cellular activity -e.g. structural protein of a cell, DNA and RNA polymerase, genes coding for ribosomal proteins -constitutive Regulated genes genes that can be turned on and off at a needed basis Francois Jacob and...

BMSC 210 Midterm 2 | 100% Correct Answers | Verified | Latest 2024 Version What is genetic engineering? - using in vitro techniques to alter genetic material in the lab What is recombinant DNA technology? - the artificial recombination of DNA from two organisms What are some basic techniques of genetic engineering? - DNA amplification electrophoesis nucleic acid hybridization molecular cloning expressing foreign genes targeted mutagenesis What is heterologous expression? - expr...

What are the similarities between human and bacterial DNA? - ️️double stranded, universal genetic code translation - ️️genetic information coded in mRNA directs formation of a specific protein at a ribosome in the cytoplasm 1970s - ️️DNA cloning tech tools to understand human genome genomics - ️️sequencing human genome bioinformatics - ️️molecular biology and computer science Francis Crick - ️️central dogma, DNA - RNA - protein model organisms - ️️short genera...

Biochemistry Quiz Latest Update Already Passed What is the primary role of phospholipids in cellular structures? Phospholipids form the bilayer of cell membranes, creating a barrier that separates the interior of the cell from the external environment. How do enzymes lower activation energy in biochemical reactions? Enzymes stabilize the transition state and provide an alternative reaction pathway, lowering the energy required for the reaction to proceed. What is a cofactor, an...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

What is genetic engineering? - using in vitro techniques to alter genetic material in the lab What is recombinant DNA technology? - the artificial recombination of DNA from two organisms What are some basic techniques of genetic engineering? - DNA amplification electrophoesis nucleic acid hybridization molecular cloning expressing foreign genes targeted mutagenesis What is heterologous expression? - expressing a gene in a different host What is the polymerase chain reaction? - rapid a...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

What is Molecular Biology?A hybrid discipline that combines elements of genetics and biochemistry. It is the study of gene structure and function at the molecular level (cloning methodology, RNA, DNA, how genes operate, nucleic acid chemistry). Can the earliest work on genes be considered as molecular biology or molecular genetics?no Was the molecular nature of genes known?no What is the early work on genes termed?transmission genetics, as it dealt with the transmission of traits from...