Cloning in pcr - Guides d'étude, Notes de cours & Résumés

BMSC 210 Midterm 2 | 100% Correct Answers | Verified | Latest 2024 Version What is genetic engineering? - using in vitro techniques to alter genetic material in the lab What is recombinant DNA technology? - the artificial recombination of DNA from two organisms What are some basic techniques of genetic engineering? - DNA amplification electrophoesis nucleic acid hybridization molecular cloning expressing foreign genes targeted mutagenesis What is heterologous expression? - expr...

MCB 2050 Molecular Biology of the Cell - W21 Practice Midterm exam questions and answers tips with Dr. Yankulov University of Guelph PART A 1. Which of the following is NOT an essential component of a cloning vector? a. A unique restriction endonuclease cleavage b. A dominant selectable marker gene c. An origin of replication d. A Taq polymerase binding site 2. Methylation of DNA sequence is used how by bacteria? a. Methylation of DNA can inhibit cell division b. Methyla...

Describe DNA structure - Answer-DNA forms a double stranded helix and adenine pairs with thymine, and cytosine pairs with guanine What pair forms hydrogen bonds and how many - Answer-thymine/adenine form 2 bonds guanine/cytosine form 3 bonds Explain "the central dogma" - Answer-DNA turning into RNA turning into Protein What is transcription - Answer-Copying of DNA code to RNA, one nucleotide at a time so it is complementary to the DNA What is translation - Answer-RNA gets translated int...

What is genetic engineering? - using in vitro techniques to alter genetic material in the lab What is recombinant DNA technology? - the artificial recombination of DNA from two organisms What are some basic techniques of genetic engineering? - DNA amplification electrophoesis nucleic acid hybridization molecular cloning expressing foreign genes targeted mutagenesis What is heterologous expression? - expressing a gene in a different host What is the polymerase chain reaction? - rapid a...

Tools used in biotechnology and genetic engineering - Answer-In Silico analysis, DNA microarray analysis, gel electrophoresis, PCR, and RFLP analysis. PCR - Answer-*Polymerase Chain Reaction* technique for quickly and easily making many copies of even a very small amount of DNA Reverse Transcriptase - Answer-enzyme that turns single-stranded RNA into double-stranded DNA Complementary DNA (cDNA) - Answer-DNA produced from an RNA template using reverse transcriptase. often used to clone ...

MCB3020 Exam 3 Bacusmo Questions And Answers 100% Correct Biotechnology use of organisms to form useful products Genetic engineering the deliberate modification of the organisms genetic information by directly changing the sequence of nucleic acids in its genome Recombinant DNA technology procedures used to carry out genetic engineering Cloning generation of a large number of identical DNA molecules 5 basic steps of cloning 1. isolate DNA to be cloned 2. Use restriction enzyme or PCR t...

Among its many other uses, PCR is finding important applications in early clinical diagnosis of disease. An example is in testing for the presence of viruses to which antibodies do not appear until a long time after infection. Describe how PCR could be used to detect HIV infection at an early stage, including what information about HIV would be needed to begin with. PCR could be used to detected HIV by performing PCR on cells with primers that are designed to amplify the viral HIV DNA that...

EPPN 154 Exam 3 Questions & Answers 2024/2025 Expressing foreign genes in bacteria - ANSWERSTranscription and translation to produce coded proteins Obstacles in expressing foreign genes in bacteria - ANSWERS1. Genes placed under control of a bacterial promoter 2. Introns must be removed 3. Codons might need editing 4. Many eukaryotic proteins require host modification after translation Expression Vectors allow for control of what? - ANSWERSexpression of the cloned genes How mus...

Bio 202 EXAM 3 QUESTIONS AND CORRECT DETAILED ANSWERS How is recombinant DNA technology used to clone the human GH gene? 1st step! - ANSWER 1. use PCR to obtain the gene (Simply cutting the DNA with restriction enzymes would not be an effective way to isolate a specific gene. The genome would be cut into so many fragments, if you ran a gel it would be a smear! Which fragment is yours? You would need hybridization to isolate your fragment. Wouldn't have much DNA- not like the amo...

What are the similarities between human and bacterial DNA? - ️️double stranded, universal genetic code translation - ️️genetic information coded in mRNA directs formation of a specific protein at a ribosome in the cytoplasm 1970s - ️️DNA cloning tech tools to understand human genome genomics - ️️sequencing human genome bioinformatics - ️️molecular biology and computer science Francis Crick - ️️central dogma, DNA - RNA - protein model organisms - ️️short genera...