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VIROLOGY FINAL EXAM Questions with CorrectAnswers
What enzyme must all RNA viruses encode in their genome? Which
RNA viruses must also carry this genome in their virions? Correct
Answer-All RNA viruses must encode an RNA dependent RNA
polymerase. (-)RNA viruses and ds RNA viruses must carry this genome
in their virions.
Label the eclipse period, latent period, and burst on the following graph
Correct Answer-
In a neutralization assay, what is being assessed for its ability to block
infection? What is the endpoint, or readout, of the assay? Correct
Answer-The antibody dilution at which the antibodies prevent
characteristics fo the viral infection. The antibodies are being assessed.
Name the viral structure Correct Answer-C: genome
D: capsid
E: matrix
C and D together: nucleocapsid
B: envelope
,circles on B: glycoprotein
What is the difference between incidence and prevalence? Correct
Answer-new cases divided by population in a particular time frame is
incidence and prevalence is infected individuals divided by population at
a particular time.
why are viruses obligate parasites? Correct Answer-They do not have
ribosomes so they cant make their own proteins
You have two cell lines and you want to infect one with a virus. One cell
line is susceptible but not permissive. The other cell line is permissive
but not susceptible. What is the easiest way to get a cell line that is both
susceptible AND permissive? Correct Answer-There will be multiple
blocks to the viral reproduction cycle in the first cell line. This will be
very difficult.
The easiest way to get a cell line both susceptible and permissive is
through the second cell line by finding a receptor to help get the virus in.
Once the virus is in, it has all the pieces to get infected within the cell.
Place the steps in the viral replication cycle in order: attachment, release,
assembly, entry, production of viral mRNA, genome replication,
translation Correct Answer-assembly, attachment, entry, production of
viral mRNA, translation, genome replication, release
,You have a virus which takes 1 hour to replicate. You are preforming an
experiment where you need to infect your cells with enough virus that at
70 minutes half of the cells have lysed. What MOI should you use to
infect your cells? Correct Answer-MOI is 2.17. My reasoning behind
this is that it takes the virus 1 hour to replicate which is 60 minutes. You
want half the cells lysed by 70 minutes so for the number of replication
cycles it is 70 minutes/ 60 minutes which is 1.17 cycles. Then to
calculate the MOI you need to be aware that to achieve half of the cells
lysed there needs to be at least 2 times the number of host cells. To
calculate MOI you need to do 2^1.17 which is 2.25. The approximate
MOI for this problem is 2.25
Order the five steps in membrane fusion mediated by class 1 fusion
proteins.
The dimers dissociate and resemble into trimers
Insertion of the fusion peptide in the target membrane
Movement of the globular heads of the surface subunit
Adoption of an extended hairpin confirmation
Membranes are brought into close proximity leading to membrane
fusion
Refolding of the hairpin
, Unfolding of the sandwich domain Correct Answer-1. movement of the
globular heads of the surface subunit
2. Adoption of an extended hairpin confirmation
3. Insertion of the fusion peptide in the target membrane
4. Refolding of the hairpin
5. Membranes are brought into close proximity leading to membrane
fusion
Match each viral RNA synthesis paradigm to the corresponding genome
type
Genome
(+) RNA
(-) RNA
ds DNA
Paradigm
stop and start synthesis
within particle synthesis
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