ASCP Exam Questions & Answers 2023/2024
Which is required to generate cDNA
A. DNA polymerase
B. RNase H
C. Restriction Enzyme
D. Helicase - ANSWER-B
What is the function of Taq Polymerase - ANSWER-recognize dsDNA
You have a 50 μL primer stock at 9pM/μl and want dilute the stock to ...
What is the function of Taq Polymerase - ANSWER-recognize dsDNA
You have a 50 μL primer stock at 9pM/μl and want dilute the stock to 900nM. How much stock should
you add(what is the dilution factor) - ANSWER-2
What gene causes hypersensitivity to Irinotecan? - ANSWER-UGT1A
Which allele causes hypersensitivity to Carbamazepine? - ANSWER-HLA-b*1502
What gives MLPA its specificity - ANSWER-Stuffer Sequence
How is copy number determined? - ANSWER-MLPA
What is the gene that cause resistance to Vancomycin - ANSWER-vanA
Which assay is FISH best used for - ANSWER-Prader Willi
RNA polymerase II: where is it and what does it do - ANSWER-Eukaryotic: It catalyzes the transcription of
DNA to synthesize precursors of mRNA and most snRNA and microRNA. (transcribes mRNA)
,Hazard that is blue and is level 4 what does it mean - ANSWER-big health hazard
Dr calls wanting test results but results have not been signed out by a medical director what do you do -
ANSWER-don't give out the result until it has been signed out my the MD
What trinucleotide repeat is involved in Fragile X? - ANSWER-CGG
In Fragile X, what is considered a full mutation expansion? - ANSWER-200+
What method is used to detect Fragile X? - ANSWER-Southern blot (now PCR /mPCR)
Which gene is the largest and has deletions/duplications as the most common mutation types -
ANSWER-DMD
Which HCV geneotype(s) has/have the best prognosis - ANSWER-Genotypes 2 &3
Positive attributes of bDNA - ANSWER-Can be used for both DNA and RNA
What type of primer set is best? - ANSWER-forward primer should complement DNA strand and reverse
primer should mirror and inversely complement the forward primer
What are some things that stabilize RNA? - ANSWER-Inactivated and minimizing exposure to
environmental/endogenous RNases
mRNA: 5'cap
What is the concentration of DNA with an OD of 2.0 - ANSWER-100 μg/mL
, Given the following data what can quantifiably be said about DNA when 260 = 1.4 and 280 = 1.1 -
ANSWER-There is 70ng of pure DNA
Sample is contaminated with proteins and phenols
What is the equation for PCR efficiency - ANSWER-(1+Σ)^n
You have a gel where the positive control worked, the negative control worked, patient 1 had a double
band, patient 2 was positive, and patient 3 had no bands. What would you do? - ANSWER-repeat patient
3
How do you detect for methylation - ANSWER-Bisulfate sequencing
MSI (Micro-Satellite Instability) tests for what? - ANSWER-Mismatch Repair Genes
Translocation at t(15, 17) - ANSWER-Translation at t(15, 17)
Coumadin can be affect by a mutation in what gene - ANSWER-CYP2C19-KVORC1
What virus interfere with kidney transplant patients - ANSWER-BK
You have 2 HIV viral load tests 5 days apart 1 being high on day one and 1 being low by day 5. What do
you do? - ANSWER-Look into storage and transport
You are given a strand of primers. Calculate the Tm - ANSWER-2˚(AT) + 4˚(GC)
When doing a multiplex you observe faint bands. What do you do - ANSWER-Adjust the annealing temp
What do you do when you want to optimize a PCR reaction that generates short PCR fragments? -
ANSWER-Adjust annealing temperature
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