Purificati - Study guides, Class notes & Summaries

PURIFICATION OF HEXAHISTIDINE TAGGED GFP PROTEIN FROM TRANSFORMED E.COLI XL1 CELLS USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY Lee Jia Yi (12) Abstract In this study, a recombinant histidine-tagged green fluorescence protein(His6-GFP) was produced by E.coli XL1 cells followed by immobilized metal affinity chromatography (IMAC) using Nickel-agarose beads for purification. The GFP template was first amplified from pE-GFP vector using polymerase chain reaction (PCR). The primers c...

LAB 9 - Green Fluorescent Protein (GFP) PurificationWhat is a protein? Proteins are long chains of amino acids, some of which are hydrophobic Explain the relationship between genes and proteins: Genes contain the genetic code which determine the amino acid composition of a protein. There is a unique gene for each protein within all of the cells of the body Using your own words, describe cloning: The duplication and propagation of a cell or organism. Describe how the bacte...

Water Purification Exam 2024 with complete solution

Water Purification UPDATED ACTUAL Exam Questions and CORRECT Answers Water - CORRECT ANSWER- The most important potential common source of infectious disease (i.e., Cholera, Shigellosis, Typhoid fever, etc); Can also be a source for chemically induced intoxications Public health - CORRECT ANSWER- Ensuring water purity is essential for _______ Microorganisms - CORRECT ANSWER- Treatment and purification schemes use _____ to identify, remove and degrade pollutants

IDENTIFICATION OF RECOMBINANT GFP PROTEIN USING CLONING, EXPRESSION AND PURIFICATION Abstract. 144 Green fluorescent protein is an useful tool to observe the presence of protein-protein interaction as well as gene expression(Chalfie et al., 1994). Which those experiment include amplification and recombination of GFP open reading from using RT-PCR which can clone GFP and insert into pQE30 vector with IPTG(Riedy et al., 1995). which is used to promote expression of N terminal hexahis...

PURIFICATION OF GFP FUSION PROTEINS WITH IMAC IN E. Coli CELLS ABSTRACT This report was performed to demonstrate techniques of purifying Green Fluorescent Protein (GFP). The main technique used for demonstration is by Immobilized Metal Affinity Chromatogrpahy (IMAC). The open reading frame for enhanced green fluorescent protein was subcloned into the pQE30 expression. The subsequent production of protein tagged N-terminally with hexahistidine, facilitated the purification by IMAC

Water Purification Exam Questions and Answers 100% Solved Water - The most important potential common source of infectious disease (i.e., Cholera, Shigellosis, Typhoid fever, etc); Can also be a source for chemically induced intoxications Public health - Ensuring water purity is essential for _______ Microorganisms - Treatment and purification schemes use _____ to identify, remove and degrade pollutants Wastewater - Domestic sewage or liquid industrial waste Wastewater treatment - Relie...

A water source with a pH value less than what number is an indication of possible CBR contamination? - Answer-6 In a driven well, the sections of well pipe are delivered in lengths of what size? - Answer-5 feet A temporary water source should not be converted into a permanent water source until after what activity has taken place? - Answer-An area search for a source requiring less development A well that is hand dug usually 3 feet in diameter or more is within what depth range? - Answe...

When you are using copper sulfate to treat a lake, concentrations of organisms should never exceed how many parts per million to protect the lives of fish? - Answer-2 ppm A water source with a PH value less than what number is an indication of possible CBR contamination? - Answer-6.0 What are two classifications of water impurities? - Answer-Suspended or dissolved When jetting a well, you turn the jet or grill slowly for what purpose? - Answer-To ensure the hole is straight The pump ...

BIOS 136 Purification exam