Chromatography - Study guides, Class notes & Summaries
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AQA A-level BIOLOGY 7402/2 Paper 2 Version: 1.0 Final *jun237402201* IBQUESTION PAPER & MARKING SCHEME/ [MERGED] Mark scheme June 2023/M/Jun23/E13 7402/2
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AQA 
A-level 
BIOLOGY 
7402/2 
Paper 2 
Version: 1.0 Final 
*jun* 
IB/M/Jun23/E13 7402/2 
For Examiner’s Use 
Question Mark 
1 
2 
3 
4 
5 
6 
7 
8 
9 
10 
TOTAL 
Friday 16 June 2023 Morning Time allowed: 2 hours 
Materials 
For this paper you must have: 
• a ruler with millimetre measurements 
• a scientific calculator. 
Instructions 
• Use black ink or black ball-point pen. 
• Fill in the boxes at the top of this page. 
• Answer all questions. 
• You must answer the questions in...
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AAMC MCAT Practice Exam 2 Questions and Answers 2024
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AAMC MCAT Practice Exam 2 Questions and Answers 2024 
C/P: What expression gives the amount of light energy (in J per photon) that is converted to 
other forms between the fluorescence excitation and emission events? 
"intensity of fluorescence emission at 440 nm excitation at 360 nm) was monitored for 20 
minutes" 
A) (6.62 × 10-34) × (3.0 × 108) 
B) (6.62 × 10-34) × (3.0 × 108) × (360 × 10-9) 
C) (6.62 × 10-34) × (3.0 × 108) × [1 / (360 × 10-9) - 1 / (440 × 10-9)] 
D) (6.62 × 10...
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BTEC Applied Science Unit 2C- Chromatography (Distinction)
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Exemplar assignment for the third assignment of Unit 2 in BTEC Applied Science, Unit 2C, which is about different chromatography techniques and their uses. If you take anything from this assignment, please put it in your own words otherwise it will count as plagiarism! Also do not hesitate to message me if you have any questions about this assignment.
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BTEC Applied Science Unit 2 Assignment C - Chromatography
- Essay • 17 pages • 2022
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BTEC Applied Science Unit 2 Assignment C - Chromatography. 
Pictures, graphs, tables included. 
Referenced throughout. 
Awarded a DISTICTION.
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CHEM 212 Lab 2 Report Separation of Organic Compounds –Column Chromatography
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CHEM 212 Lab 2 Report Separation of Organic Compounds –Column Chromatography 
 
CHEM 212 Lab 2 Report Separation of Organic Compounds –Column Chromatography
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BIOCHEMISTRY LAB REPORT 4 2023/2024| PORTAGE LEARNING|WITH PROCEDURES, EXPLANATION AND FINAL RESULTS.HIGHLY VERIFIRD Experiment #: Lab 4 Title: Chromatography of molecules.
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BIOCHEMISTRY LAB REPORT 4 2023/2024| PORTAGE LEARNING|WITH PROCEDURES, EXPLANATION AND FINAL RESULTS.HIGHLY VERIFIRD 
 
 
Experiment #: Lab 4 
 
Title: Chromatography of molecules.
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PURIFICATION OF HEXAHISTIDINE TAGGED GFP PROTEIN FROM TRANSFORMED E.COLI XL1 CELLS USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY
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PURIFICATION OF HEXAHISTIDINE TAGGED GFP PROTEIN FROM TRANSFORMED 
E.COLI XL1 CELLS USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY 
Lee Jia Yi 
 (12) 
Abstract 
In this study, a recombinant histidine-tagged 
green fluorescence protein(His6-GFP) was 
produced by E.coli XL1 cells followed by 
immobilized metal affinity chromatography 
(IMAC) using Nickel-agarose beads for 
purification. The GFP template was first 
amplified from pE-GFP vector using 
polymerase chain reaction (PCR). The primers 
c...
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Unit 2 - Learning aim C: Chromatography
- Essay • 12 pages • 2022
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Unit 2 - Learning aim C: Chromatography 
 
Contains: 
 
P5: Correctly use chromatography techniques to procedure chromatograms. 
 
P6: Explain the use of chromatographic techniques to separate mixtures 
 
M3 - Analyse own chromatograms + relate factors that affect separation of mixtures to quality of results obtained 
 
 
D3 - Evaluate chromatographic techniques used in relation to outcomes + suggest improvements 
 
 
D3 - Evaluate chromatographic techniques used in relation to outcomes + sugge...
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applied science year 1 unit 2c - chromatography
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distinction grade assignment for unit 2c chemistry (chromatography) in year 1 of applied science / forensic and criminal investigation btec
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Chem 241 Chromatography Questions And Answers Latest Update
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What are the benefits of having smaller particles in HPLC? - correct answers high efficiency, higher plate number, higher pressure, shorter run time, better resolution due to uniform flow (reduced A-term, multiple pathway) 
 
what is plate height? - correct answers length of column required for 1 equilibration of solute between mobile and stationary phases 
 
What are the drawbacks of having smaller particles in HPLC? - correct answers lower detection limit, resistance to solvent flow, increased...
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Chem 241 Exam 2: Chromatography Questions And Answers Latest Update 2024
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History of chromatography (ch 23) - correct answers -Greek word "chromatus" for color 
-Tswett (1903) first separated plant pigments 
 
Applications - correct answers Breast milk, water analysis, urinalysis for drug doping 
 
Goals of separation - correct answers Recovery of product, purity of product 
 
Type, SP, MP - correct answers 1. Adsorption: one polar substance sticks to another polar substance. Solid SP, gas or liquid MP 
2. Partition: dissolving of 2 similar liquids. Liquid SP, gas o...
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