Sodium dodecyl sulfate - Study guides, Class notes & Summaries

Matching A) electrophoresis B) hydrophobic interaction C) enzyme-linked immunosorbent assay D) three-dimensional shape E) N-terminal amino acid F) negative charge G) nucleases H) chromophore I) foaming J) high level expression K) 2-mercaptoethanol L) positive charge M) cation exchange N) pI O) chymotrypsin P) C-terminal amino acid Q Sodium dodecyl sulfate 1. One of the reasons the primary structure is important for a protein is that it determines the ______ the molecule adopts...

Henderson-Hasselbach Equation - pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography - Separates sample based on size with smaller molecules eluting later. Ion-Exch...

ACS Biochemistry Final Exam Questions and Correct Solutions 2024 pKa (chapter 3) - Answer -measure of the tendency of a group to give up a proton (acidity); tendency decreases tenfold as pKa increases by one unit thioester bond (1) - Answer -compounds with functional group C-S-CoA-C (eg, in acetyl-CoA) zwitterion (3) - Answer -dipolar ion with both positive and negative groups but overall neutral charge; can act as either an acid or base Isoelectric point (pI) (chapter 3) - Answer -the ch...

pKa (chapter 3) - measure of the tendency of a group to give up a proton (acidity); tendency decreases tenfold as pKa increases by one unit thioester bond (1) - compounds with functional group C-S-CoA-C (eg, in acetyl-CoA) zwitterion (3) - dipolar ion with both positive and negative groups but overall neutral charge; can act as either an acid or base Isoelectric point (pI) (chapter 3) - the characteristic pH at which the net electric charge is zero SDS - Sodium Dodecyl Sulfate (chap ...

Chapter 5: Proteins: Primary Structure Matching A) electrophoresis B) hydrophobic interaction C) enzyme-linked immunosorbent assay D) three-dimensional shape E) N-terminal amino acid F) negative charge G) nucleases H) chromophore I) foaming J) high level expression K) 2-mercaptoethanol L) positive charge M) cation exchange N) pI O) chymotrypsin P) C-terminal amino acid Q Sodium dodecyl sulfate 1. One of the reasons the primary structure is important for a protein is that it de...

SDS, which stands for sodium dodecyl sulfate, is an anionic _______; it may break down _______ bonds. - right answer--detergent; dipole-dipole You are conducting a gel electrophoresis to determine small differences in protein subunit molecular weights. Which of the following is a valid reason to choose a polyacrylamide gel over an agarose gel for your purposes? - right answer--Polyacrylamide has a higher resolution than agarose and will be able to distinguish smaller differences in sizes. ...

WGU C001 BIOCHEMISTRY STUDY GUIDE QUESTIONS WITH VERIFIED ANSWERS Henderson-Hasselbach Equation - ANSWER pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis - ANSWER Used in synthesis of a growing amino acid chainto a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) - ANSWER Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatog...

BIO 361 Exam 1, Summer 2020 Exam Answer Key An annotated version with answer explanations will be available in the next few days 1. You have observed that many of your colleagues who are studying enzymes keep them in an ice bucket, rather than at room temperature. You have just discovered that the enzyme you are studying appears to unfold when chilled, but it remains active at room temperature or somewhat above. An explanation for your observation could be: a. the deltaH for unfolding of...

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Henderson-Hasselbach Equation pH = pKa + log ([A-] / [HA]) FMOC Chemical Synthesis Used in synthesis of a growing amino acid chain to a polystyrene bead. FMOC is used as a protecting group on the N-terminus. Salting Out (Purification) Changes soluble protein to solid precipitate. Protein precipitates when the charges on the protein match the charges in the solution. Size-Exclusion Chromatography Separates sample based on size with smaller molecules eluting later. Ion-Exchange Chr...