Bch5413 - Study guides, Class notes & Summaries

MAMMALIAN GENETICS BCH5413 COMPLETE EXAM ...

BCH5413 EXAM 1 STUDYGUIDE ...

BCH5413 LECTURE 1 AND 2 TEST LATEST UPDATE...

In the development of knockout mice, it is critical to genotype the mice to determine if the gene of interest is actually knocked out. Below is a figure for the primer locations for triplex PCR. Which of the following PCR results would represent true knockout mice? - Answer One band of relatively lower molecular weight * The primer pair for the knockout gene produces a smaller fragment than the endogenous gene. If both genes are replaced with PGK-NEO, PCR will produce one band of a relati...

What is the difference between and intron and an exon? - Answer Introns are non- coding sections and exons are coding sequences. How can one stretch of DNA be used to create more than one protein? - Answer It can undergo alternative splicing and create different protein products. What is the meaning of the term exon shuffling? - Answer Exon shuffling is when an exons from different genes are brought together or the same exon is duplicated.

BCH5413 TESTED QUESTIONS WITH REVISED ANSWERS Type of information found in a cDNA library - Answer-The genes that are expressed in a particular tissue or cell type. cDNA is reverse transcribed from the RNA that was expressed in a tissue or cell type. the purpose of electromobility shift assay... - Answer-determine if a specific protein binds to a specific DNA in vitro If a protein of interest binds to a labeled oligonucleotide, it will increase the molecular weight of the fragment and shi...

In the development of knockout mice, it is critical to genotype the mice to determine if the gene of interest is actually knocked out. Below is a figure for the primer locations for triplex PCR. Which of the following PCR results would represent true knockout mice? - Answer One band of relatively lower molecular weight * The primer pair for the knockout gene produces a smaller fragment than the endogenous gene. If both genes are replaced with PGK-NEO, PCR will produce one band of a relati...

DNA is negatively charged and will move to the positive side the smaller travel more quickly *rate inversely proportional to their length What is used to view DNA in gel - Answer ethydium bromide Sournces of dsDNA - Answer - eukaryotic genome bacterial genome plasmid viral genome

BCH5413 Exam One 2024 with Questions and 100% Correct Answers

BCH5413 TEST 2 QUESTIONS AND ANSWERS ALL REVISED AND UPDATED Function of clamp loader - ask whether loader is catalytic or loads one clamp per DNA and then stops - Answer-- circular ssDNA + primer + clamp/Pol subunit - with increasing amount of clamp loader, increasing synthesis of DNA is seen *result shows that it functions catalytically (one loader can load clamp onto 10 molecules of template) Function of clamp loader - ask whether loader remains associated with pre-initiation complex or...