MIC 206

Transmission of Disease correct answer: 1. Conact 2. Vehicle 3. Vector - Contact between infected and uninfected person - Sexual contact or kissing - Touching, biting or scratching - PLACENTA - Exit or Entry correct answer: Examples of Direct Contact - Pathogen transmitted via inanimate object (fomite) - Needles, toothbrushes, money, diapers, drinking glasses, utensils - Object is contaminated, then uninfected person touches it correct answer: Example of Indirect Contact - ...

What is the purpose of staining? correct answer: to add contrast for visibility What are color-bearing ions referred to as? correct answer: chromophores What kind of ions do basic stains have? correct answer: positive charges What is the process called that you do to a MO on a slide before staining it? correct answer: smear prep What are the steps to a smear prep? correct answer: draw circle --> flip and label --> dab 2x loops of MO --> spread --> air dry --> heat...

3 cell shapes correct answer: bacilli, cocci, sprillium by adding colored compound, we add ________ for visibility correct answer: contrast basic stains are __________ correct answer: cations methelyne blue, safranin, and crystal violet are _____ stains correct answer: basic acidic stains are ______ correct answer: anions Heat fix in front of the _____________ for 5 seconds correct answer: bacticinerator when switching to high power and oil power __________ move the coars...

Phenylalanine Deaminase Test correct answer: Input(s) = amine group Output(s) = pyruvic acid, NH2 Enzyme(s) = phenylalanine deaminase Reagent(s) = 10% FeCl3 pH Indicator = (+) avocado green, (-) clear Principle of Test: - Identifies organisms that can synthesis the enzyme Phenylalanine Deaminase - Deamination reactions remove the amine (N) groups, producing compounds that are acidic. Starch Hydrolysis Test correct answer: Input(s) = starch Output(s) = glucose Enzyme(s) = amyl...

ability to transfer microbes from one place to another without contaminating the original culture, the new medium or the environment correct answer: define aseptic technique - disinfecting workbenches before and after use/washing hands - sterilizing media and inoculating instruments - transfer of microbes without contamination - pinky finger hold of test tube cap, tilting the test tube to minimize air contaminants, working efficiently to reduce to exposure to air, etc. correct answer: lis...

Simmons Citrate Utilization Test correct answer: Determines the ability of an organism to use sodium citrate as its sole carbon source Simmons Citrate Inputs and Outputs correct answer: Inputs - sodium citrate outputs - basic products Simmons Citrate pH indicator correct answer: Bromthymol Blue Simmons Citrate Enzyme correct answer: Citrate permease Simmons Citrate possible results correct answer: Positive - blue Negative - green Malonate Test correct answer: organism's a...

Simmons citrate test: enzyme correct answer: citrate permease Simmons citrate test: pH indicator correct answer: Brothymol blue Results of a positive Simmons citrate test correct answer: Turns blue can use sodium citrate as sole carbon source Results of a negative Simmons citrate test correct answer: Stays green color --> pH doesn't change doesn't contain the enzyme Malonate test: enzyme correct answer: Succinate dehydrogenase What does the Malonate test do? correct an...

Membrane Filter Method correct answer: Selective and differential Media: Fuschin sulfiote indicator to identify lactose fermenters will appear dark red in color All non lactose fermenters will appear colorless Calculate the coliforms CFU per 100ml Countable colonies for membrane filter method correct answer: 20-200 Mold is correct answer: Eukaryotic Aerobic Cell wall has chitin Chemoheterotrophs Filamentous Sporangia Major divisions are based in their sexual reproductive ...

Types of media correct answer: complex, defined, selective, differential Complex media correct answer: Unknown chemical makeup Wide range of growth Defined media correct answer: Known chemical makeup Low range of growth Selective media correct answer: Has a component added that will inhibit or prevent growth of certain of certain types of bacteria or will promote growth of desired species Differential media correct answer: Component is added to distinguish between different ty...

what is a simple stain and what does it allow for correct answer: A basic stain that is rinsed with water that allows one to identify cell morphology (shape) and arrangement three cell shapes correct answer: bacillus, cocci, spirillum what do stains contain that allow staining to occur correct answer: they contain chromophores, color bearing ions by adding colored compounds in staining, we add ___ for visibility of cells correct answer: contrast charge of bacterial cells correct ...