CMB2001 Questions and Answer | Latest Update 2024/2025
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Course
CMB2001
Institution
CMB2001
Describe how H3Lys9me heterochromatin is formed
~~> H3Lys9me heterochromatin formation: Lys9 deacetylated by
HDAC. Suvar39 then methylates lys9. Methylated lys9 is recognised by
HP1 and then HP1 binds. HP1 has chromodomain - chromodomains
often recognise and bind to methylated lys residu...
✓ ~~> Encode proteins whose levels can be easily measured eg GFP
and LacZ
Amount of reporter protein is proportional to gene expression (activity)
Reporter genes can be added to plasmids which cause recipient cells to
have reporter gene expression
Contrast types of RNA pol in prokaryotes and eukaryotes
✓ ~~> In prokaryotes there's only one
In eukaryotes there are at least 3 main ones
Similar structure but bacterial has 2 alpha, 1 beta, 1 beta prime and one
omega subunit. Yeast RNA pol II has 12 subunits.
What are types of RNA pol in eukaryotes?
✓ ~~> RNA pol I - transcribe rRNA (28S, 18S and 5.8S). Found in nucleolus
Master01 | September, 2024/2025 | Latest update
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