CMB2001 L01 Questions and Answer | Latest Update 2024/2025
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Course
CMB2001
Institution
CMB2001
Consensus sequences are described with IUPAC ambiguous nucleotide
codes.
W is A or T
S is G or C
R is G or A
Y is T or C
What is the span of the core/basal region of the eukaryotic promoter?
~~> -30 to +30nt
What elements are in the core region of the eukaryotic promoter?
~~> TATA...
What is observed in core promoter CpG islands without obvious TATA and Inr
sequences?
✓ ~~> Lower transcription rate, initiation at multiple start sites
What is the difference between a distal and proximal regulatory promoter
element?
✓ ~~> Proximal = within 1kb of the start site
Distal = 1kb or more away from the start site
How do you identify promoter elements via sequence comparison?
✓ ~~> Analyse base frequency within many genes to compare and
identify a consensus sequence
How do you identify promoter elements via reporter analysis?
Master01 | September, 2024/2025 | Latest update
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