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BOC Study Guide Histotechnology 2nd ed.- Fixation (HT)
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BOC Study Guide Histotechnology 2nd ed.- Fixation (HT)
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BOC Study Guide Histotechnology 2nded.- Fixation (HT)
Kidney biopsy tissue has been fixed in phosphate-buffered
glutaraldehyde for 2 hours and then placed in phosphate
buffer solution. If a portion of this tisssue is processed for
light microscopy, sections would most likely show: - Correct
Answer ✅nonspecific PAS staining
Uric acid crystals are preserved ONLY when tissue is fixed in: -
Correct Answer ✅absolute alcohol
Improper preservation of tissue will result if there is: -
Correct Answer ✅a delay in fixation
A good fixative will: - Correct Answer ✅protect tissue
against alteration during subsequent processing.
The function of methanol in commercial formalin solutions is
to: - Correct Answer ✅retard the polymerization of
formaldehyde
In electron microscopy, Zamboni fluid, glutaraldehyde, and
osmium tetroxide function as: - Correct Answer ✅fixative
solutions
Tissue will remain unfixed if placed in: - Correct Answer
✅sodium borate
,BOC Study Guide Histotechnology 2nd
ed.- Fixation (HT)
Bouin Solution is contraindicated for: - Correct Answer
✅tissue to be stained by the Feulgen reaction
Formalin pigment can be removed from tissue sections by
treatment with 10%: - Correct Answer ✅ammonium
hydroxide in 70% alcohol
Stock neutralized formalin is prepared in the laboratory by
storing the solution over a layer of calcium carbonate. The
solution withdrawn from this stock container will: - Correct
Answer ✅become acidic
Microscopic evaluation reveals a poorly stained h&E section
of spleen. These results will be difficult to remedy if the
problem is: - Correct Answer ✅poor fixation
An example of an additive fixative is one that contains: -
Correct Answer ✅picric acid
Microscopic examination of an H&E stained section fixed in
formalin shows marked nuclear bubbling. One most often
sees this artifact if the specimen is processed following: -
Correct Answer ✅incomplete fixation
, BOC Study Guide Histotechnology 2nd
ed.- Fixation (HT)
Microscopic evalution of H&E stained sections from a
surgically removed small bowl specimen shows an absence of
much of the epithelium in otherwise normal tissue. This most
likely resulted from: - Correct Answer ✅delayed fixation
When compared with tissue fixed in formalin, tissue fixed in
zinc-formalin will show: - Correct Answer ✅superior nuclear
detail
A certain project requires a fixative that contains acetic acid
yet stabilizes erythrocyte membranes. One fixative that could
be used is: - Correct Answer ✅Hollande solution
A specimen of kidney must be shipped to another city for
immunofluorescence studies. The specimen should be placed
in: - Correct Answer ✅Michel Solution
When the microwave oven is used for fixation, the most
critical factor is the: - Correct Answer ✅control of
temperature
To adequately remove the calcium from a specimen
containing areas of microcalcification, the tissue should be
fixed in: - Correct Answer ✅Hollande solution
Which of the following fixatives contains copper acetate? -
Correct Answer ✅Hollande
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