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Molecular & Cellular Biology Lab Final Exam: Questions With Solutions
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Molecular & Cellular Biology Lab Final Exam: Questions With Solutions
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Molecular & Cellular Biology Lab Final Exam:Questions With Solutions
Which of the following is one of the unintended effects of transfection?
Right Ans - Both A and B
Which one of the following is not a method/reagent for transfection: Right
Ans - Detergent
The contractile properties of ------------ is crucial for retraction Right Ans -
myosin II
What stage follows the polarization stage seen in cell migration? Right Ans
- protrusion
In TA cloning, the cloning of PCR products takes advantage of the the tendency
of Taq Polymerase , which often adds an extra _____________ to the 3' end of the
product. Right Ans - adenine
What are the functions or Potassium ion and Ammonium ion in the PCR buffer
respectively Right Ans - K+ promotes primer annealing; NH4+has a
destabilizing effect, thus enhancing specificity
Adenoviruses Right Ans - Complex double-stranded DNA viruses that can
infect both dividing and non-dividing cells but are non-integrating.
During a DNA miniprep, Ethanol (EtOH) is added during a miniprep for what
reason? Right Ans - Ethanol precipitates the DNA from the rest of the
miniprep solution.
We incubate the newly transformed cells for 1 hour at 37C before plating the
bacteria on LB/Kanamycin media. Why is this not necessary for ampicillin
resistant plasmids? Right Ans - Kanamycin blocks protein synthesis. If we
were to plate the cells before incubation nothing would grow due to the lack
of the necessary proteins needed for colony growth. Need more info
, If one wanted to make a bacteria that would grow in the presence of
ampicillin, what would be the most appropriate part of the plasmid to
consider? Right Ans - bacterial antibiotic resistance gene
If you wanted to select some mammalian cells to keep expression of your
plasmid of interest (stable cell line), what would be the most appropriate part
of the plasmid to consider? Right Ans - selectable marker
When is the best time to write down procedures and observations in a lab
book? Right Ans - as experiments are done
Why must we incubate the newly transformed cells for 1 hour at 37C before
plating the bacteria on LB/KAN media? Right Ans - KAN blocks protein
synthesis. If we were to plate the cells on LB/Kan media before we incubated
the cells, then we would not see any growth due to the lack of necessary
proteins which were prevented from forming by the KAN.
What enzyme(s) is used during the process of transfection? Right Ans -
None of these
At what temperature should E. coli be incubated? Right Ans - 37° C
What kind of plasmid would you use if you wanted to copy a piece of DNA to
move to another plasmid? Right Ans - a cloning plasmid
The application of an electrical current across a cell membrane resulting in
temporary "pore" formation enabling the uptake of exogenous molecules
found in the medium to either the cytoplasm (transient transfection) or into
the nucleus (stable transfection) is: Right Ans - Electroporation
What is the advantage of electroporation over other transfection
methodology? Right Ans - The ability to introduce materials into many
different kinds of cells and organisms.
In order to make stable cell lines it is essential to have a plasmid that contains
a: Right Ans - selectable marker, such as an antibiotic resistance gene.
What are the steps in generation of stable cell lines incorporating plasmid
DNA? Right Ans - Selecting stable transfected cells
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