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UCI BIO 93 EXAM QUESTIONS WITH VERIFIED ANSWERS
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UCI BIO 93 EXAM QUESTIONS WITH VERIFIED ANSWERS....
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What about Chargaff's Rule is true? [A] = [T] and [G] = [C] are the answers.Which bond—A-T or G-C—is more difficult to break? ANSWER Because
there are three H-bonds between G and C (guanine and cytosine), the bond
between them is more difficult to break.
However, between A and T, there are just two H-bonds (adenine and thymine).
What did the Griffith Experiment's findings suggest? ANSWER When a
pathogenic strain of bacteria is mixed with a nonpathogenic strain that is still
alive, some of the living cells may change into the pathogenic form.
Which element is absent from DNA but present in proteins? ANSWER Sulfur
Which element is absent from proteins but present in DNA? ANSWER
Phosphorus
What is the "genetic material" according to the findings of the Hershey and
Chase experiment? ANSWER DNA, since the pellet contained ³²P.
42 percent of the nucleotides in an organism's DNA sample are composed of
cytosine. What proportion of the nucleotides in this sample will be thymine,
roughly speaking? Thymine (ANSWER 8%). This is due to the fact that
A+T+C+G equals 100%. If there is 42% C, then there is roughly 42% G
because C base pairs with G. 84% of the total bases are made up of 42% C and
42% G. After that, we get 16% (100%) minus 84%. The two remaining bases
(A and T) receive 8% apiece when 16% is divided by them.
Describe the Griffith experiment's use of bacterial transformation and its
definition. ANSWER A change in genotype and phenotype brought on by a
cell's assimilation of foreign DNA is known as bacterial transformation. The
Griffith experiment demonstrated that genetic material from living
, nonpathogenic cells and heat-killed pathogenic cells may be transferred. Even
though the pathogenic cells were dead, the mouse died when injected with this
combination, suggesting that the genetic material must have been transmitted to
the nonpathogenic cells that were still alive. The investigation also revealed the
existence of living, multiplying harmful cells in the blood sample.
Two students chose to replicate the Hershey and Chase experiment, albeit with
some changes, for a science fair project. Instead of labeling the DNA's
phosphate, they chose to name its nitrogen. Will this initiative succeed? Why
not? ANSWER Since protein and amino acids both include nitrogen atoms, this
project will fail. Therefore, radioactivity would not be able to tell DNA from
protein.
ANSWER --- Lecture #7
What is molecular biology's core tenet? DNA --transcription--> RNA is the
answer. --translation--> protein.
Translation takes place in the cytoplasm, whereas transcription takes place in
the nucleus.
Which of the following DNA sequences is complementary? ANSWER 3'-TCC
GCT ACG TGA-5' 5'-AGG CGA TGC ACT-3'
Which DNA replication model is semiconservative? ANSWER In order for
free nucleotides to attach to the complementary nucleotide, a parental DNA
molecule divides into two strands. DNA splits into two daughter molecules that
are exactly same.
The Meselson-Stahl experiment: what was it? ANSWER bacteria grown in a
medium containing the heavy isotope ¹⁵N. Bacteria were then moved to a
medium containing ¹⁴N (lighter isotope).
Results: A layer of the ¹⁵N isotope was visible in the center of the tube when the
DNA sample was centrifuged following initial replication. A layer of ¹⁴N
isotope was visible above the first layer of ¹⁵N in the DNA sample that was
centrifuged following the second replication.
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