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Bio – 219 Questions With Complete Solutions

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  • BIO 219

Bio – 219 Questions With Complete Solutions How long is the lux operon 8.5 kb long A. fischeri Bacteria found throughout marine environments Auto-induced bioluminescence An operon that allows A. fischeri to glow under the right conditions Vector types Plasmids Phagemids Bacteriophage Vect...

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  • November 12, 2024
  • 16
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • BIO 219
  • BIO 219
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Bio – 219 Questions With Complete Solutions

How long is the lux operon 8.5 kb long




A. fischeri Bacteria found throughout marine environments




Auto-induced bioluminescence An operon that allows A. fischeri to glow under the right

conditions




Vector types Plasmids


Phagemids

Bacteriophage Vectors

Cosmid Vectors

Bacteria/Yeast artificial chromosomes




Phagemids phage + plasmid


-package ssDNA in presence of a helper phage




Bacteriophage Vectors Based on the lambda virus

,Bio – 219 Questions With Complete Solutions
larger capacity >25 kb per virus




Cosmid Vectors lambda cos sites + plasmid


->35 kb (35-45kb) inserts availabe

-can be packed in viral proteins for infectivity

-low copy number in bacteria




Bacterial/yeast artificial chromosomes -large, low copy number 1-2 per cell


->300 kb inserts are acceptable

-Good for sequencing




We chose phagemids because it will operate as a plasmid




its small circular DNA

-1-200kb

-can accept DNa fragments from 1-10kb

-extra-chromosomal

, Bio – 219 Questions With Complete Solutions
Self replicating




Exist in nature to carry antibiotic resistance and other survival genes




can be transmitted between two bacteria




been engineered into excellent vectors




What makes a good plasmid vector? Size


-large enough to hold workable quantities of foreign DNA

-Small enough to be retained by the host

-small enough to be able to distinguish from host chDNA




High copy number

-50 to 100 per cell




Origin of replication (ORI)

- Must be recognizable by the host's replication machinery

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