BI 127 Lab @ GRCC Exam 2 (labs 11-14) Questions With Complete Solutions
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Course
GRCC
Institution
GRCC
BI 127 Lab @ GRCC Exam 2 (labs 11-14)
Questions With Complete Solutions
if you want to raise the pH of a medium, what would you do? add a strong base
"floaties" at the bottom of a tube will indicate what? growth
a ring around the top of a tube will indicate what? growth
what does fastidious me...
BI 127 Lab @ GRCC Exam 2 (labs 11-14)
Questions With Complete Solutions
if you want to raise the pH of a medium, what would you do? add a strong base
"floaties" at the bottom of a tube will indicate what? growth
a ring around the top of a tube will indicate what? growth
what does fastidious mean? the organism depends on the environment to deliver ready-
made organic compounds
which kind organisms are the most fastidious and which are the least? autotrophs are least
fastidious
hetertrophs are most fastidious (even the least fastidious heterotroph is more fastidious than the
most fastidious autotroph)
what is a serial dilution? taking defined amounts of a liquid and adding it to another tube,
taking a defined amount of that liquid and so on; this will dilute the concentration with each
, BI 127 Lab @ GRCC Exam 2 (labs 11-14)
Questions With Complete Solutions
transfer (as long as the amount transferred is consistent the dilution should be a factor of 10...)
Then transfer from specific tubs by adding a small amount to an agar plate >> allows us to
monitor growth (want between 30-300 CFU's)
what is the formula for original cell density? # colony forming units
---------------------------
original sample volume
or
# CFU
-------------------
(10^#) x volume transferred
what words are used to define the shape of a colony? round
, BI 127 Lab @ GRCC Exam 2 (labs 11-14)
Questions With Complete Solutions
irregular
filamentous
rhizoid
what does an irregular shaped colony look like? looks like mountains from above in a
plane
what does a filamentous colony look like? like a frozen surface; like small branches but
not root-like
what does a rhizoid colony look like? big roots or branches coming off - continue
branching off of other branches
what are the words to describe different margins of a colony? (margin = edge of a colony
and what it's like on top)
smooth/entire
rhizoid
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