after successful bacterial transformation with plasmid DNA, you plan to isolate the
plasmid DNA from the genomic DNA using a miniprep. what should you not do? -
answer use water to precipitate and wash DNA
how would you describe a tertiary protein structure? - answer the three-dimensional
shape of the polypeptide chain once is folded
what is true about protein synthesis? - answer transfer RNA (tRNA) molecules that carry
amino acids
you observe an unknown cell under a microscope. a nucleus is visible. what kind of cell
is it? - answer eukaryotic
under normal physiological conditions, the cell's plasma membrane prevents exogenous
nucleic acids from entering the cell. this is because the membrane is: - answer
impermeable to charged substances
what diffuses from the plant shoot tips to cause cell differentiation? - answer hormone
what is the gram stain procedure in order? - answer crystal violet as stain, iodine as
mordant, alcohol as decolorizing agent, gram safranin as counter stain
in the ABO blood type system, what is the universal donor? - answer type O
describe t-cells - answer process and present antigens on their cell surfaces
describe b-cells - answer produce antibodies
describe antibodies - answer bind and label pathogens
to start the process of transcription, RNA polymerase binds to the ________________
to initiate mRNA synthesis - answer operon
utilizing a patient's own cells to produce a novel drug or medical treatment is called? -
answer personalized medicine
, monoclonal antibodies are derived from the fusion of tumor cells and B cells, which are
then grown in cell culture. what is this technique called? - answerhybridoma technology
what technique would you use to distinguish a GMO-containing food from a non-GMO-
containing food? - answerPCR
golden rice is a rice variety genetically engineered to produce beta-carotene, a
precursor of vitamin A. what is not a benefit? - answerencouraging crop biodiversity
what is an ethical use of genetic screening? - answerdeveloping personalized medical
treatments
prior to testing in humans, a new drug candidate is tested for safety what regulatory
practices? - answercGLPs
what is correct in regards to clinical trials? - answerthe emphasis of a Phase I clinical
trial is on treatment safety
When a co-worker wears nitrile groves out of the lab, what is the appropriate action? -
answernotify the co-worker to not wear gloves outside the lab
you notice a tear in the packaging of a disposable loop that you are about to use for an
experiment. what is the correct response? - answerdiscard. the content and packaging
is compromised and the risk of contamination is high
what is a technique for harvesting bacteria from overnight liquid culture? -
answerstreaking
when is it acceptable to aseptically transfer bacteria without wearing gloves? - answerit
is never acceptable
steam sterilization occurs using what? - answerhigh temperature
a microscope has 4X ocular lens and 10X objective. what is the microscope's total
magnification? - answer40X
what does the lysis step in a plasmid purification do? - answerbreaks open the bacterial
wall
for a bacterial chromosomal DNA extraction, what is the optimal growth phase to
harvest the cells? - answerearly growth phase
how would you describe agarose gels? - answerprimarily used for separating DNA
fragments, and are usually run horizontally
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