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BACE Review (Technical Skills & Applications) Questions and Answers Latest Updated
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BACE Review (Technical Skills & Applications)
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BACE Review (Technical Skills &Applications)
Which of the following should not be used to disinfect an area that is potentially
contaminated with DNA? - answer Alcohol
Why are enzyme solutions always prepared using a buffered solvent at a specific pH? -
answer A buffered solvent is resistant to fluctuations in pH because it is made with a
combination of a weak acid and its conjugate base. Enzymes perform best in solutions
that vary in pH.
Which of the following statements best illustrates the reason for separation of DNA
during gel electrophoresis? - answer DNA fragments are separated in the gel matrix
based on their size.
Which of the following should be included in a gel electrophoresis to determine the size
of the DNA fragment on the gel? - answer Molecular Weight Markers
Gels are used to separate pieces of RNA, DNA, and protein. What type of gel is
typically used for protein electrophoresis? - answer Polyacrylamide
A plasmid has three restriction sites for EcoRI. If the plasmid is treated EcoR1, how
many fragments will be obtained? - answer3
Which of the following can be used as a vehicle for delivering new DNA into a cell? -
answer Plasmid, Lipid and Virus
When developing a new PCR procedure, every variable needs to be tested to determine
the optimum conditions for that specific reaction. Which of the statements best
describes the best experimental design for optimizing the primer-annealing
temperature? - answer Multiple reactions run with Primer, DNA, dNPT's, and a buffer
concentration kept constant, only changing the annealing temperature in a range of 50
to 70 degrees Celsius.
Which of the following enzymes are used to amplify DNA in a Polymerase Chain
Reaction (PCR)? - answer DNA Polymerase
Heat-Stable DNA Polymerase - answer Polymerase Chain Reaction (PCR)
Restriction Enzymes - answer Digesting DNA
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