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BACE Practice. Technical Skills and Applications Questions and Answers 2024
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BACE Practice. Technical Skills and Applications
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BACE Practice. Technical Skills andApplications
PCR function - answer to locate and amplify a gene or some other target sequence from
a sample of DNA
PCR reagents - answer Water, buffer, dNTPs, Taq Polymerase, Primers, gDNA
dNTPs - answer free nucleotides (As, Ts, Cs, Gs), ready to be polymerized
PCR Steps - answer1. Denaturation
2. Annealing
3. Extension
DNA ligase - answer enzyme that repairs breaks in the sugar-phosphate backbone.
phosphodiester bond - answer covalent bonds between sugars and phosphates in DNA
and RNA.
Column Chromatography function - answer to isolate something from a mixture
How Column Chromatography Works - answer The chemical you are trying to separate
binds to the column matrix. The other solutes are washed out. Then you add a solution
that your chemical is most attracted to and it releases/eluted from your column.
Charge of DNA - answer Negative due to phosphate groups
How 260nm/280nm is done - answer A spectrophotometer is used to measure the
absorbance of a DNA solution. The absorbance at 260nm is divided by the absorbance
at 280nm. If the ratio is between 1.8 and 2.0, the solution has very high purity.
SDS - answer Chemical that causes proteins to unravel/denature. It also sticks to the
proteins, causing them to become negatively-charged.
SDS-PAGE – answer Poly-Acrylamide Gel Electrophoresis. Gel electrophoresis for
proteins. Separated proteins according to size.
Acrylamide - answerA gel polymer used in SDS-PAGE
Coomassie Blue - answera dye that stains proteins blue (so you can see them in a
PAGE gel). You add the stain after you run the gel.
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