Exam (elaborations)
BACE - Technical Skills & Applications Questions and Answers
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BACE - Technical Skills & Applications
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BACE - Technical Skills & ApplicationsWhy must technicians practice aseptic technique when culturing cells? - answer To
ensure they are growing pure cultures of their desired cells.
Which of the following techniques destroys all microorganisms? - answer Sterilization
When using a microscope on the highest power objective, which focus knob should you
use? - answer Fine
Staining with Coomassie Blue - answer To visualize proteins after PAGE
Adding a molecular weight standard to an empty well - answer To determine the relative
sizes of the samples run on the gel
Adding SDS to the sample buffer and the PAGE running buffer - answerTo coat the
sample proteins with a negative charge, and denature them
Adding loading dye to protein samples - answerTo facilitate loading samples into wells,
and to monitoring sample migration during PAGE
In recombinant DNA technology, DNA ligase is used to rejoin which of the following
bonds in DNA? - answerPhosphodiester
A restriction enzyme digestion reaction contains which of the following reagents? -
answerDNA, Water, Buffer, Restriction Enzyme
PCR - answerused to amplify specific DNA sequences
Chromatography - answera commonly used technique to separate proteins from
complex mixtures
Gel-Filtration/Size Exclusion Chromatography (SEC) - answerSeparates proteins based
on size
Hydrophobic Interaction Chromatography (HIC) - answerSeparates proteins based on
solubility in water
Affinity Chromatography - answerSeparates proteins based on unique functional groups
Ion Exchange Chromatography (IEC) - answerSeparates proteins based on charge
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