Gel Electrophoresis Quiz (Questions + Answers) Solved
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Course
Gel Electrophoresis
Institution
Gel Electrophoresis
What would you expect to happen if you left the gel accidentally in the gel
electrophoresis chamber for too long? - ️️the DNA strands would not stop they
would continue to move through the gel into the buffer
In what situation do scientists need to use the PCR reaction? - ️️to amplify a
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Gel Electrophoresis Quiz
What would you expect to happen if you left the gel accidentally in the gel
electrophoresis chamber for too long? - ✔️✔️the DNA strands would not stop they
would continue to move through the gel into the buffer
In what situation do scientists need to use the PCR reaction? - ✔️✔️to amplify a
specific sequence or make many copies of a particular fragment
What causes the DNA fragments to move within the gel? - ✔️✔️The liquid buffer
solution helps the electricity flow when an electric current is applied to the solution in the
gel. DNA has a negative charge so it attracts the positive electrode gel and moves
through
Which DNA fragments move faster? Which slower? - ✔️✔️Smaller DNA fragments
move faster, while longer DNA strands move slower
What causes the normally colorless DNA to glow under UV light? - ✔️✔️Ethidum
Bromide
What tool is used to transfer small liquid solutions of DNA from tubes into the gel? -
✔️✔️Micro pipette
What chemicals and reactants go into the tube before it goes in to the PCR machine? -
✔️✔️primer, Dna strand, water, spare nucleotides, enzyme
What is the PCR machine called? - ✔️✔️Thermal cycler
What is the purpose of the RNA primers in the reaction? - ✔️✔️the RNA primers mark
the ends of the segment they want to study and tell the reaction what DNA fragment to
copy
Why are the spare nucleotides needed in the reaction? - ✔️✔️they are needed to
create the copied DNA
What would you expect would happen if the scientist forgot to put the DNA copying
enzyme in the tube before putting the tube in the machine? - ✔️✔️DNA wouldn't be
copied
Why do scientists use restriction enzymes? - ✔️✔️they cut DNA at specific sequences
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