Histology for CSMLS Exam Questions And
Answers 100% Pass
3 types of Carbohydrates demonstrated in tissues - answer✔Glycoproteins, polysaccharides,
proteoglycans
What stain is used for glycogen? - answer✔PAS - because glycogen has vicin diols
Where is glycogen found in the body? - answer✔Liver, skeletel and cardiac muscle, hair follicles, vaginal
and ectocervical epithelium, mesothelium cells, neutrophils and megakaryocytes.
What accounts for the staining reaction of Glycoproteins? - answer✔sialic acid goups and vicin diols
(PAS), also contains carboxyl acid group (Alcian Blue).
Where are glycoproteins in the body? - answer✔Most are associated with epithelial tissues. They are
classified as either neutral or acidic depending on whether they contain a Sulphate or Carboxylate
residues.
What do neutral glycoproteins lack? Where do you see them? - answer✔They lack bother Carboxylate
(COOH) and Sulphate (SO3H). They are found in goblet cells in the stomach as well as paneth cell
granules in the small bowel.
Where are acidic glycoproteins that contain only COOH found in the body? - answer✔They can be found
submaxillary gland, small intestine, fetal extracellular matrix, upper part of colonic crypts, sublingual
gland.
Where are acidic glycoproteins that contain both COOH and SO3H found in the body? - answer✔Mainly
found in colonic goblet cells.
What are Proteoglycans (Glycosaminoglycans)? - answer✔They are large, unbranched carbohydrates
with small poly peptide chains.
Where in the body can you find Proteoglycans? - answer✔Connective tissues such as cartilage, bone,
tendons, ect. Referred to as acid or neutral mucins.
What compound reacts with Cationic dyes in a Carbohydrate? - answer✔The acid containing sugar
(COOH or SO3H)
What are the routine stains for Carbohydrates ? - answer✔PAS, Alcian Blue Muricarmine, Colloidal iron,
Metachromasia, Lectins
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