Gel Electrophoresis Questions and Answers Graded A+
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Course
Gel Electrophoresis
Institution
Gel Electrophoresis
Why do gel electrophoresis? - ️️1. To determine which organisms are most closely
related.
2. To determine paternity of a child.
3. For crime scene analysis.
Bands - ️️A DNA ladder usually contains a set of known DNA fragments with
different sizes in base pairs (bp) or kilobases (kb). T...
Gel Electrophoresis Questions and
Answers Graded A+
Why do gel electrophoresis? - ✔️✔️1. To determine which organisms are most closely
related.
2. To determine paternity of a child.
3. For crime scene analysis.
Bands - ✔️✔️A DNA ladder usually contains a set of known DNA fragments with
different sizes in base pairs (bp) or kilobases (kb). These DNA fragments are separated
and visualized as DNA bands on a gel.
What is gel electrophoresis for? - ✔️✔️It is used to separate DNA fragments based
upon size.
First step in gel electrophoresis - ✔️✔️DNA is first cut using special enzymes called
restriction enzymes.
Second step in gel electrophoresis - ✔️✔️The DNA sample is loaded into an agarose
gel.
Third step in gel electrophoresis - ✔️✔️An electric charge is applied to the gel. The
negatively charged DNA moves toward the positive side of the gel.
Gel electrophoresis - ✔️✔️A technique used to separate segments of DNA based on
their size using an electric current
Wells - ✔️✔️The spaces that are loaded with DNA samples
Restriction Enzyme - ✔️✔️Cuts DNA molecules at a specific base sequences (either
STR or VNTR) to make smaller fragments
Longer DNA fragments - ✔️✔️Move slower through the gel and end up closer to the
wells.
Shorter DNA fragments - ✔️✔️Move faster through the gel and end up further from the
wells .
DNA ladder - ✔️✔️These separated DNA bands together look like a ladder on the gel.
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