Exam (elaborations)
Bio 219 Final Exam Review Questions And Answers Latest Update
- Course
- BIO 219
- Institution
- Purdue University
Bio 219 Final Exam Review Questions And Answers Latest Update
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Bio 219 Final Exam ReviewQuestions And Answers Latest
Update
Two main goals of project Answer: 1. Isolate the lux operon out of A.fischeri
and use it to transform E.coli to induce bioluminescence
2. Create a restriction map of a lux-containing plasmid (OR detect GadA
expression levels)
Bioluminescent bacterial organism used? Answer: A.fischeri
Explanation of symbiotic relationship between this organism and hosts Answer:
A.fischeri gets glucose and amino acids from host, host can camouflage from
predators, attract prey, or attract mates
Two marine organisms that take part in this symbiotic relationship Answer:
Hawaiian bobtail squid and Angler fish
Lux operon: Definition, Size, Components and their Responsibilities Answer:
Lux operon induces bioluminescence in A. fischeri
8.5kb long
luxI-encodes for auto-inducer
luxR-encodes for transcriptional activator that binds to auto-inducer
luxC-acyl-reductase enzyme
, luxD-acyl-transferase enzyme
luxA-alpha subunit of luciferase (has active site)
luxB-beta subunit of luciferase (necessary for activity of enzyme)
luxE-acyl-protein synthetase enzyme
Biochemical Rxn that causes Bioluminescence Answer: •FMNH2 + O2 + R-
CHO à FMN + R-COOH + H2O + LIGHT
Luciferase catalyzes light emitting rxn
Acyl-transferase removes fatty acids from normal pathway for use in rxn, acyl-
protein synthetase activates fatty acid to form R-CO-AMP (use ATP), acyl-
reductase reduces activated fatty acid to form necessary aldehyde (use NADPH)
Quorum sensing Answer: Regulation of gene expression in response to
changes in cell-population density
Auto-induction Answer: Bacteria release AHLs (N-acyl homoserine lactone) to
freely diffuse into extracellular environment. As bacteria density increases, more
inducer is released. When a certain concentration is hit, inducer diffuses back into
cells to interact with Lux operon (turn on gene expression)
E. coli LuxR homolog? Answer: SdiA
Can E.coli use quorum sensing? How do they do this in nature Answer: Can
use quorum sensing from other organisms that produce AHLs as a means to up or
down regulate parts of its genome
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