100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Bio 219 Final Exam Review Questions And Answers Latest Update $14.49   Add to cart

Exam (elaborations)

Bio 219 Final Exam Review Questions And Answers Latest Update

 1 view  0 purchase

Bio 219 Final Exam Review Questions And Answers Latest Update

Preview 2 out of 14  pages

  • November 7, 2024
  • 14
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
All documents for this subject (10)
avatar-seller
Schoolflix
Bio 219 Final Exam Review
Questions And Answers Latest
Update

Two main goals of project Answer: 1. Isolate the lux operon out of A.fischeri
and use it to transform E.coli to induce bioluminescence
2. Create a restriction map of a lux-containing plasmid (OR detect GadA
expression levels)


Bioluminescent bacterial organism used? Answer: A.fischeri


Explanation of symbiotic relationship between this organism and hosts Answer:
A.fischeri gets glucose and amino acids from host, host can camouflage from
predators, attract prey, or attract mates


Two marine organisms that take part in this symbiotic relationship Answer:
Hawaiian bobtail squid and Angler fish


Lux operon: Definition, Size, Components and their Responsibilities Answer:
Lux operon induces bioluminescence in A. fischeri
8.5kb long
luxI-encodes for auto-inducer
luxR-encodes for transcriptional activator that binds to auto-inducer
luxC-acyl-reductase enzyme

, luxD-acyl-transferase enzyme
luxA-alpha subunit of luciferase (has active site)
luxB-beta subunit of luciferase (necessary for activity of enzyme)
luxE-acyl-protein synthetase enzyme


Biochemical Rxn that causes Bioluminescence Answer: •FMNH2 + O2 + R-
CHO à FMN + R-COOH + H2O + LIGHT
Luciferase catalyzes light emitting rxn
Acyl-transferase removes fatty acids from normal pathway for use in rxn, acyl-
protein synthetase activates fatty acid to form R-CO-AMP (use ATP), acyl-
reductase reduces activated fatty acid to form necessary aldehyde (use NADPH)


Quorum sensing Answer: Regulation of gene expression in response to
changes in cell-population density


Auto-induction Answer: Bacteria release AHLs (N-acyl homoserine lactone) to
freely diffuse into extracellular environment. As bacteria density increases, more
inducer is released. When a certain concentration is hit, inducer diffuses back into
cells to interact with Lux operon (turn on gene expression)


E. coli LuxR homolog? Answer: SdiA


Can E.coli use quorum sensing? How do they do this in nature Answer: Can
use quorum sensing from other organisms that produce AHLs as a means to up or
down regulate parts of its genome

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller Schoolflix. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $14.49. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

80796 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 14 years now

Start selling
$14.49
  • (0)
  Add to cart