Genetic Technology Self-Assessment
Quiz Complete Answers
In the paternity test results below, which children did Dad father? - Answer-D1 and S1
Which of the following lists the steps of producing a DNA profile in the correct order? -
Answer-Isolate DNA from body fluid samples; amplify selected genetic markers with
PCR; run the amplified DNA on a gel; compare the patterns of DNA bands on the gel
Which of the following could be a goal of genetically engineering a bacterium? -
Answer-a and b
What is PCR (polymerase chain reaction)? - Answer-a method of amplifying DNA
How would a plasmid be made that could introduce a gene into a bacterium? - Answer-
the same restriction enzyme would be used to prepare the source DNA and cut open
the plasmid; the source DNA and opened plasmids would be mixed together; DNA
ligase would be added to permanently incorporate the source DNA into the plasmid.
What is an RFLP? - Answer-restriction fragment length polymorphism
What is the biggest obstacle to genetic engineering in plants and animals? - Answer-
introducing foreign genes into eukaryotic cells
What is DNA profiling? - Answer-an analysis of DNA fragments produced by using a
restriction enzyme
What is a cDNA? - Answer-a copy of a DNA gene that lacks introns
In transformation, new genes are transferred into bacteria when - Answer-bacteria pick
up DNA from the solution around them, usually in the form of a plasmid
What is an STR? - Answer-short tandem repeat
What is an RFLP? - Answer-a pattern of fragment lengths produced by a specific
restriction enzyme
What is reverse transcription? - Answer-a method that can produce a eukaryotic DNA
gene that does not contain introns
Which of the following is a problem posed by DNA profiling? Choose all that apply. -
Answer-How can invasion of privacy be prevented by the formation of genetic data
banks?
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