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Cambridge A Level Genetic Technology Exam Questions and Answers

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  • Course
  • Genetic Technology
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  • Genetic Technology

Cambridge A Level Genetic Technology Exam Questions and Answers

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  • November 7, 2024
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  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • Genetic Technology
  • Genetic Technology
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Cambridge A Level Genetic Technology
Exam Questions and Answers
Genetic engineering - Answer-any procedure in which genetic information in an
organism is changed by altering the nucleotide base sequence of a gene to create a
GMO.

GMO - Answer-a genetically modified organism that has its DNA changed in a way that
does not occur naturally or by selective breeding.

Recombinant DNA - Answer-DNA made by artificially joining together pieces of DNA
from two or more different species.

Transgenic organism - Answer-any organism that contains DNA from another source,
including another organism of the same or different species.

Vector - Answer-a means of delivering genes into a cell used in gene technology (e.g.
plasmids or viruses).

Restriction endonuclease - Answer-an enzyme derived from bacteria that cuts DNA
molecules at particular sequences (usually palindromic ones) to form specific DNA
fragments.

Bacteriophage - Answer-a virus with double stranded DNA that infects bacteria.

Gene probe - Answer-a length of DNA that has a complementary base sequence to
another piece of DNA that you are trying to detect.

Genome - Answer-the complete set of genes and all genetic material present in a cell or
unicellular organism (includes genetic material inside membrane bound organelles).

Promoter - Answer-a length of DNA that includes a binding site for RNA polymerase
(and transcription factors in eukaryotic DNA) so that transcription of a gene or complex
of genes can begin.

Gene editing - Answer-deleting, inserting or replacing a length of DNA within a gene
using a method such as the Crispr/Cas9 system.

PCR - Answer-polymerase chain reaction which amplifies selected regions of DNA
using alternate stages of polynucleotide separation and DNA synthesis.

gel electrophoresis - Answer-a process used to separate charged molecules (e.g. DNA
fragments) using an electric field depending on their relative mass.

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