Biology 16.2 The Process of Genetic
Engineering Exam Questions and
Answers
Using the PCR process, just a few dozen cycles of _______ can produce billions of
copies of the DNA between the _____ in less than one day. - Answer-replication,
primers
When a sample contains too little DNA to be analyzed easily, scientists use a technique
known as ______ ______ ______ to make multiple copies of specific DNA sequences. -
Answer-polymerase chain reaction
Short pieces of single-stranded DNA of about 20 or 30 bases, known as ______, are
introduced to the small sample of DNA. - Answer-primers
The base sequences of ______ are chosen to be ______ to sequences on either end of
the region of DNA to be copied. - Answer-primers, complementary
PRC step 1: The DNA sample is _____ to separate its ____ . - Answer-heated, strands
PRC step 2: As the DNA cools, the ____ bind to the ____ strands. - Answer-primers,
single
PRC step 3: DNA ______ copies or replicates the region between the _______ . -
Answer-polymerase, primers
PRC step 4: As the cycle of heating and cooling is repeated, the copies serve as
______ to make still more copies. - Answer-templates
Scientists can take DNA molecules from any source and insert them into living cells by
using _____ ______ such as DNA ligase to splice them together. - Answer-restriction
enzymes
The "sticky ends" left by restriction enzymes allow DNA fragments to combine. These
combined molecules are known as ______ ______. - Answer-recombinant DNA
Recombinant DNA is produced by recombining _____ from different _____. - Answer-
DNA, sources
_______ DNA technology makes it possible to change the ______ composition of living
organisms. - Answer-Recombinant, genetic
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