Portage Microbiology Module 4 Exam Questions and Correct Answers Latest Update
growth media - Answers nutrient-rich environment for isolating and culturing microorganisms - liquid or solid
LB media (lysogeny broth) - Answers Most common nutrient Broth
Known for ability to grow vast array of mic...
growth media - Answers nutrient-rich environment for isolating and culturing microorganisms - liquid or
solid
LB media (lysogeny broth) - Answers Most common nutrient Broth
Known for ability to grow vast array of microbes
Selective Media - Answers allows for growth of specific microbes, while restricting the growth of other
microbes
often used to grow human pathogens, or slower growing microbes. The selectivity allows for discounting
foreign unwanted microbes
Differential Media - Answers allows growth of several types of microbes and displays visible differences
among those microbes
ex. both gram(-) ecoli v salmonella as ecoli ferments lactose and growth w lactose shows red, where
salmonella remains white/tan
Enriched Media - Answers used to grow fastidious orgs - complex growth requirements that will cease to
grow if not exact.
Agar - Answers Polysaccharide derived from seaweed extract
Solid & smooth allowing growth of colonies in isolated dots
Covering entire plate = lawn
more agar = more firm/
LB agar - Answers Lysogeny broth (LB), a nutritionally rich medium, is primarily used for the growth of
bacteria; LBagar capable of wide variety of micro'o's.
LB is non-selective and non-differential.
Trypticase Soy Agar - Answers TSA or TSAYE - identical in color to LBagar
Multipurpose media w high variety
Non-selective & non-differential
Blood Agar - Answers BAPlates, derivative of TSA combined with mammalian blood, at [5-10%]
, BAP are red
BAP rich, non-selective & are differential
RBC allow for growth of fastidious micro'o's IE strep
Allows for detection of HEMOLYTIC ACTIVITY
BAP Hemolysis - Answers Alpha h- greenish-browish color, due to incomplete/partial lysis of RBC
Beta h- capacity a microbe has to completely lyse RBC
Gamma h- lack of hemolytic activity, resulting colonies often white/tan growing on red background of
agar plate
Columbia CNA agar - Answers Red color, enriched selective and differential
Used for isolation of gram(+) bact
Media suppresses growth of Gram(-) bact = contains antimicrobials colistin and nalidixic acid
Chocolate Agar - Answers CHOC, or CBA Chocolate Blood Agar
enriched, non-selective, non-differential derivative of BAP
Plate contains RBC that have been lysed via heat PRIOR to plating (differs BAP as those are lysed on the
plate)
The cooking of RBC gives dark choc color
Lysed red cells release various growth factors into media required for cultivation of fastidious,
pathogenic bact.
Enriched
examples: Haemophilus influenzae and Neisseria Meningitidis -- gram (-)
MacConkey Agar (MAC) - Answers -*Selective* for *gram-negative bacteria*. Inhibits the grow of gram-
positive bacteria and fungi w crystal violet and bile salts
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