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PHMY 610 GRAM NEG AEROBES PT 2 EXAM 4 QUESTIONS AND ANSWERS
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PHMY 610 GRAM NEG AEROBES PT 2 EXAM 4 QUESTIONS AND ANSWERS...
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PHMY 610 GRAM NEG AEROBES PT 2 EXAM 4QUESTIONS AND ANSWERS
with non-lactose fermentors, what is the next step for differentiation
oxidase test
this test is used to identify bacteria that produce cytochrome c oxidase, an
enzyme of the bacterial ETC
oxidase test
what is the most clinically important non-lactose fermenter
Pseudomonas spp.
which Pseudomonas spp. is the most important
Pseudomonas aeruginosa
are Pseudomonas spp. obligate aerobes or facultative anaerobes
obligate aerobes
T/F: Pseudomonas spp. can grow virtually in any environment and LOVE moist
environments
-Found in soil, water, decaying matter, vegetation
-Moist hospital environments (sinks, ventilators, toilets)
true
what are the three types of water-soluble pigments on agar that Pseudomonas
spp. may show
pyocyanin (blue)
pyoverdin (yellow-green)
pyorubin (reddish-brown)
what are some characteristics of Pseudomonas spp. to differentiate for diagnosis
mucoid appearance due to thick polysaccharide capsule (alginate), beta-
hemolytic on blood agar, grape-like smell, spreading border, metallic sheen
, what patients may Pseudomonas spp. be found in due to alginate bioflim
CF patients
Pseudomonas spp. are non-lactose fermenters, oxidase _____ and catalase
_______
oxidase positive and catalase positive
what are virulence factors for Pseudomonas spp.
adhesins, toxins, and enzymes
what do flagella and pili of Pseudomonas spp. mediate
motility
what are lipopolysaccharides of Pseudomonas spp. responsible for
endotoxin activity
what is alginate
mucoid polysaccharide of Pseudomonas spp. that forms capsule on bacterial
surface
what does alginate protect the Pseudomonas spp. from
phagocytosis and antibiotic mediated killing
T/F: Gene controlling production of alginate can be activated in patients with
CF or chronic respiratory disease who are exposed to long-term colonization
with mucoid strains
true
what does exotoxin A of Pseudomonas do
disrupt protein synthesis
what does pyocyanin & pyoverdine of Pseudomonas do
form toxic oxygen compounds
what do elastases of Pseudomonas do
degrade elastin --> damage to elastin containing tissues, lung parenchymal
damage
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