Exam (elaborations)
PHMY 610 BACTERIAL PROPERTIES TEST QUESTIONS AND ANSWERS
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PHMY 610 BACTERIAL PROPERTIES TEST QUESTIONS AND ANSWERS...
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PHMY 610 BACTERIAL PROPERTIES TESTQUESTIONS AND ANSWERS
what does acid fast mean
bacteria resist acid wash
what structure of mycobacterium causes it to be acid-fast
mycolic acid of the outer cell wall
what is the process of acid-fast testing
stain everything pink --> wash with acid alcohol --> counterstain blue
what colour would an organism be if acid-fast
pink
what colour would the organism be if non-acid-fast
blue
what is the process of gram staining
1. stain with crystal violet dye/iodine
2. wash the slide with alcohol
3. counter stain with red/pink safranin
what color will gram positive bacteria be with gram stain test
purple
what causes gram positive bacteria to stain purple
due to thick peptidoglycan cell wall layer soaking up all the initial purple dye
what color will gram negative bacteria be with gram stain test
red/pink
what causes gram negative bacteria to stain red/pink
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