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BIO 300 L EXAM 1 2024/2025 WITH 100% ACCURATE SOLUTIONS
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BIO 300 L EXAM 1 2024/2025 WITH 100% ACCURATE SOLUTIONS
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BIO 300 L EXAM 1 2024/2025 WITH 100%ACCURATE SOLUTIONS
This dye forms a complex with proteins. When the dye binds to protein
it changes color, which means that it absorbs light at a different
wavelength. - Answer Bradford Dye
An instrument that measures the proportions of light of different
wavelengths absorbed and transmitted by a pigment solution from the
Bradford dye. At 595 nm absorption is linear over a specific and narrow
range of 10-100 ug protein. - Answer Spectrophotometer
Provides reference for measuring the amount of protein in a solution of
unknown concentration. It is constructed by measuring the absorption of
several known concentrations of protein in the range of 10-100ug. -
Answer Standard Curve
What concentration is in range of the standard curve? - Answer 10-
100ug
A useful strategy for dealing with unknown reducing by one order of
magnitude with each dilutions. - Answer Serial dilutions
What was the name of the solution with known concentration in the
protein assay? - Answer Bovine Serum Albumin (BSA)
, What was the concentration of the SDS/EDTA? - Answer 1 mg/mL
What color was the dye/protein complex in the protein assay? - Answer
Blue
What do you need to do with the spectrophotometer before measuring
the assay? - Answer Blank it!
What was the absorbance set to one the spectrophotometer? - Answer
A595
How much of each solution should go in the four test tubes in the protein
assay? - Answer In the first test tube add 0.1mL of unknown and 0.9mL
SDS/EDTA, for the following tubes add 0.1mL from the tube before and
from the last tube remove 0.1mL.
What type of dilution did we perform in the protein assay? - Answer 10-
fold
How can the number of cells in suspension be collected? - Answer
Centifugation
How much E. coli was put into each 2 mL centrifuge tube? - Answer
1.8mL
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