WGU Biochemistry Final Exam |
Questions with 100% Correct
Answers
molecule that will amplify during PCR - ✔ ✔ template DNA
enzyme that synthesizes the new DNA molecule during PCR - ✔ ✔ DNA polymerase
building blocks of DNA needed for DNA synthesis during PCR - ✔ ✔ dNTPS
initiates synthesis of the new DNA molecule during PCR - ✔ ✔ Primers
why is it necessary to use heat stable DNA polymerase for PCR - ✔ ✔ heat
inactivates most enzymes. using heat stable DNA polymerase will eliminate
need to add fresh enzyme after each cycle of heating.
job of DNA polymerase in PCR - ✔ ✔ synthesizes new DNA strand
job of thermocycler in PCR - ✔ ✔ machine that cycles through different
temps needed
Is DNA ligase used in PCR - ✔ ✔ NO
this ligates Okazaki fragments
is RNA primer used in PCR - ✔ ✔ NO
initiates DNA replication
,job of dNTPS in PCR - ✔ ✔ Building blocks of DNA. needed for DNA synthesize
job of DNA primers in PCR - ✔ ✔ initiates DNA synthesize
Determining the correct primer in PCR - ✔ ✔ Always goes from 5-3 and 3-5
Is Huntington's disease dominant or recessive? - ✔ ✔ dominant
Carriers are represented. how - ✔ ✔ Ee
codominance - ✔ ✔ A condition in which both alleles for a gene are fully expressed
~has both objects presented in the off spring
complete dominance - ✔ ✔ a relationship in which one allele is
completely dominant over another
off spring will only be one of the colors
incomplete dominance - ✔ ✔ A pattern of inheritance in which two alleles,
inherited from the parents, are neither dominant nor recessive. The resulting
offspring have a phenotype that is a blending of the parental traits.
~off spring is neither object of the parents
Is osteogenesis imperfecta autosomal dominant or recessive? - ✔ ✔
autosomal dominant
, which can not be detected using PCR - ✔ ✔ epigenetic changes
correct direction in polymerase chain reaction - ✔ ✔ denaturation->
annealing-> elongation
DNA polymerase can synthesize new strands in which direction - ✔ ✔ 5' to 3'
components used in PCR - ✔ ✔ DNA polymerase
thermocycler
dNTPS
DNA primer
components used in replication - ✔ ✔ DNA polymerase
DNA ligase
RNA primer
dNTPS
Centra dogma - ✔ ✔ DNA -> RNA -> Protein
tips on how to solve genetic equations - ✔ ✔ 1. what is the relationship
between the two sequences
~identical
(coding/nontemplate) and/or (template/noncoding)
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