MCB 2610 Lab Exam I Questions and
Answers
what is the quickest and most economical method of bacterial isolation? - ANSWER-streak plate
method
what is the purpose of aseptic technique? - ANSWER-maintain pure cultures
what is the purpose of a T-streak? - ANSWER-isolate and maintain single speci...
what type of stain is used for simple staining? - ANSWER✔✔-simple crystal violet stain
if the chromophore is a positive ion, the stain is considered a(n) ______________________ stain -
ANSWER✔✔-basic
if the chromophore is a negative ion, the stain is considered a(n) __________________ stain -
ANSWER✔✔-acidic
what is the mordant used in the gram stain and what does it do? - ANSWER✔✔-iodine - it makes the
color permanent
bacteria that retain the Gram stain are classified as Gram _____________ - ANSWER✔✔-positive
what do Gram positive bacteria have that allows them to retain the primary stain? - ANSWER✔✔-thick
wall of peptidoglycan
why are Gram negative bacteria easy to decolorize? - ANSWER✔✔-they have an outer membrane and a
thinner layer of peptidoglycan
do Gram negative stains retain the counterstain (safranin)? - ANSWER✔✔-yes
why is the spore stain used with bacteria such as Bacillus and Clostridium? - ANSWER✔✔-they develop
an endospore in response to certain environmental conditions such as depletion of nutrients
T/F heat used during staining causes sporulation - ANSWER✔✔-FALSE
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