MCB 2610 Exam 1 Microscopy Study
Guide Solutions
numerical aperture - ANSWER-ability of the lens to gather light - depends on the property of the
lens and the refractive index of the medium
refractive index - ANSWER-how much a substance bends a light ray
immersion oil - ANSWER-oil placed on a ...
parfocal (microscopes) - ANSWER✔✔-remain in focus when objectives are changed
dark field microscope - ANSWER✔✔-- image is made by light reflect or refracted by specimen
- produces a bright image of the object against a dark background
- used to observe unstained preparations
- uses a hollow cone of light
phase contract microscope - ANSWER✔✔-- coverts slight differences in refractive index and cell density
into easily detected variations in light intensity
- image is produced where cells are dark and the background is light
- uses a hollow cone of light
differential interference microscope - ANSWER✔✔-- similar to phase contract where the image detects
differences in refractive index and thickness of different part of specimen
- uses two beams of polarized light to create 3D image
- can observes unstained cells that appear bright and 3D
Fluorescence microscope - ANSWER✔✔-- exposes specimen in ultraviolet, violet, or blue light
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