Genetics 133 Exam 2 UW-
Madison with 100% correct
answers 2025
Plasmid cloning vectors - answer the vehicles for amplifying specific
genes
Restriction enzymes and DNA ligase - answer the scissors and glue for
DNA cut-and-paste
• Sizing DNA fragments by gel electrophoresis - answer simple way to
identify and/or purify specific DNA fragments
PCR - answer simple, cheap, fast and universal method for amplifying any
segment of DNA (normally limited to length of 10,000 base pairs). All that
is required is knowing the sequence of the segment to be amplified.
Domestication - answer - process whereby a population of animals or
plants becomes accustomed a human controlled environment through the
process of artificial selection.
Domestication Syndrome - answer - traits characteristic of domestication
within a group of plants or animals. Example trait: loss of seed shattering
in domesticated rice, wheat barley, etc..
QTL mapping - answer method for identifying the genes responsible for a
particular trait.
Polyploidy - answer A cell or organism with three or more chromosome
sets. 3 sets = triploid, 4 sets = tetraploid, 6 sets = hexaploid, and so on.
Domestication of plants often involved an increase from diploid to triploid,
tetraploid of even hexaploidy. Increased ploidy typically increases cell and
organ size (for example, bigger apples).
Belyaev's Wild Fox Breeding Experiment - answer selection for tameness
is accompanied by unselected domestication traits (depigmentation,
floppy ears, etc.). Phenotypes begin to appear within 6 generations.
Similar results with mink and rat.
Neural Crest Hypothesis - answer - postulates that tameness evolves by
reduction of neural crest cell number, migration or function, resulting in
reduced adrenal glands (source of adrenaline "fight or flight"
neurotransmitter) along with reduction in pigmentation and cartilage/bone
(floppy ears, smaller teeth, curled tail...).
antigenic variation - answer immune evasion strategy in which it cycles
through expression of 60 var genes, each encoding a variant of a cell‐
surface protein, PfEMP1.
AIMs - answer can be used to identify parts of a genome that are
descended from particular geographical regions.
Genome Wide Association Studies (GWAS): - answer a way of identifying
genes that contribute to a trait by looking for SNPs that exhibit
statistically significant co-occurrence (i.e., association) with that trait.
Haplotype - answer a combination of SNPs inherited as a block along the
length of a chromosome.
Environmental DNA (eDNA) - answer DNA (either modern or ancient)
purified directly from environmental samples (water, soil, ice cores, etc.)
without any obvious signs or knowledge of the biological source of the
DNA.
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