SEMMELWEIS UNIVERSITY MEDICAL BIOPHYSICS I.
SEMIFINAL - PRACTICE TOPICS
How does refractive power of a lens change if its radius of curvature decreases? -
Answers-The power increases
How does refractive power of a lens change if its radius of curvature increases? -
Answers-The power decreases
What is radius of curvature in case of a lens? - Answers-Flattener lens= larger radius of
curvature. More rounded
lens=lesser radius of curvature.
What sample can be measured with the Abbe-refractometer? - Answers-Solutions with
an index of refraction lower than that of the
measuring prism (because we want snell's window)
What is the role of Amici prism? - Answers-To restore dispersed light into white light (or
vice versa)
What is optical dispersion? - Answers-Phenomenon in which white light is decomposed
into component
colors due to the dependence of refractive index on frequency
Factors influencing the value of index of refraction. - Answers-Solvent concentration in
dilute solution, the frequency, temperature
(makes fluids less viscous so light travels faster)
Formation of Snell circle. - Answers-Grazing rays from lower index of refraction medium,
refract at the boundary and make critical angles at the medium with the higher index of
refraction, the critical angles form the borders of a cone of bright light.
How do you determine concentration by refractometry? - Answers-In case of dilute
solutions n1=n0+k*c
What is the refractive index of distilled water? - Answers-1.33
Definition of absorption spectrum. - Answers-Absorbance as a function of wavelength.
Unique to every element.
What information can you obtain from an absorption spectrum? - Answers-
Concentration of solutions (based on intensity change) and the
electronic structure of the atom.
, How do you determine concentration by absorption photometry? - Answers-Beer
lamberts law: valid for dilute solutions log(Jo/J)=ext.coeff*c*xExt.coeff. depends on the
type of material and the wavelength.
Define optical density (absorbance). - Answers-=log(Jo/J)
Define transmittance. - Answers-T=J/Jo (*100%)
How much light is transmitted by a sample with an absorbance of 1? - Answers-10%
Which sample transmits more light: OD=1 or OD=3? By how much? - Answers-Shift log
^-1=1/10 (*100%)Sift log ^-3=1/1000 (*100%)OD=1 transmits one hundred-fold more
light
How does the absorption spectrum change if the sample concentration is doubled? -
Answers-It doubles (due to linear relationship in Beer Lambert's law)
How does the absorption spectrum change if the sample concentration is halved? -
Answers-It halves (due to linear relationship in Beer Lambert's law)
How does refractive power of a lens change if its index of refraction increases? -
Answers-Refractive power will increase
Calculate the refractive power of a lens with a focal distance of 25 cm. - Answers-
1/0.25=4 dpt
Calculate the refractive power of a lens with a focal distance of 20 cm. - Answers-
1/0.20=5 dpt
Calculate the refractive power of a lens with a focal distance of 17 mm. - Answers-
1/0.017=5.88 dpt
Characterize the image of an object placed within the focal distance of a converging
lens. - Answers-Virtual, magnified and upright
Characterize the image of an object placed between the single and the double focal
distance of a converging lens. - Answers-Real, magnified and inverted
Characterize the image of an object placed outside the double focal distance of a
converging lens. - Answers-Real, diminished and inverted
What kind of image is formed by a compound light microscope? - Answers-Virtual,
magnified and inverted
What is the total magnification of a light microscope if the objective magnification is
100x and the ocular magnification is 20x? - Answers-100*20= 2000
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