Exam (elaborations)
WGU C785 BIOCHEMISTRY FINAL EXAM 2022/2023 REVIEWED (LATEST UPDATE)/GRADED A+
- Course
- Institution
WGU C785 BIOCHEMISTRY FINAL EXAM 2022/2023 REVIEWED (LATEST UPDATE)/GRADED A+
[Show more]
Content preview
WGU C785 BIOCHEMISTRY FINAL EXAM 2022/2023REVIEWED (LATEST UPDATE)/GRADED A+
1. cThe cpolymerase cchain creaction cis ca ctool cused cto cstudy cprotein cstructure.
True
False c(correct canswers)False
Feedback
PCR cis ca ctool cused cto camplify ca cspecific csegment cof cDNA.
What ccolor cis cthe cprimer cin cthe cfollowing cdiagram?
Red
cPurpl
e
Blue c(correct canswers)Red
Feedback
Recall cfrom cthe csection con cDNA creplication cthat cDNA cpolymerase cneeds ca cprimer cto cbegin cDNA
csynthesis. cThis crequirement cmeans cthe cprimers cwill cdirect cthe cDNA cpolymerase cto conly
csynthesize ccomplementary cstrands cof cthe ctarget cDNA. c(Note: cIn cDNA creplication, cthe cprimers
care cRNA cprimers, cwhile cPCR cgenerally cuses cDNA cprimers cbecause cthey care cmore cstable.)
dNTPs care cDNA cnucleotides cused cin cPCR.
,True
False c(correct canswers)True
,Feedback
dNTPs cstands cfor cdeoxynucleotide ctriphosphates, cwhich care cthe cnucleotides cused cin cDNA csynthesis.
A cthermocycler cis ca cmachine cused cfor cPCR cthat cvaries cthe ctemperature cof ca csample.
True
False c(correct canswers)True
Feedback
For cPCR, cin ceach ccycle, cthe ctwo cstrands cof cthe cduplex cDNA care cseparated cby cheating, cthen cthe
creaction cmixture cis ccooled cto callow cthe cprimers cto canneal c(or cpair) cto ctheir ccomplementary
csegments con cthe cDNA. cNext, cthe cDNA cpolymerase cdirects cthe csynthesis cof cthe ccomplementary
cstrands. cThe cuse cof ca cheat-stable cDNA cpolymerase celiminates cthe cneed cto cadd cfresh cenzyme
cafter ceach cround cof cheating c(heat cinactivates cmost cenzymes). cHence, cin cthe cpresence cof
csufficient cquantities cof cprimers cand cdNTPs, cPCR cis ccarried cout csimply cby ccycling cthrough cthe
cdifferent ctemperatures cfor cstrand cseparation, cprimer cannealing, cand cDNA csynthesis.
The cthermocycler cis cthe cmachine cthat cis cused cto cvary cthe ctemperature cof cthe csamples.
DNA cpolymerase cis cused cin cDNA creplication cand cin cPCR.
True
False c(correct canswers)True
Feedback
Both cPCR cand cDNA creplication crequire cDNA cpolymerase cto cmake cnew ccopies cof cDNA.
Which cof cthe cfollowing cchanges ccan cNOT cbe cdetected cusing cPCR?
, Differences cin cDNA
csequence cEpigenetic
c changes cDeletions
Insertions c(correct canswers)Epigenetic cchanges
Feedback
Epigenetic cchanges cdo cnot caffect cthe csequence cof cthe cDNA. cPCR cis cused cto clook cat cthe cDNA
csequence.
Which cof cthe cfollowing ccomponents cis cNOT cused cin cPCR?
DNA ctemplate
cDNA
cnucleotides
cRNA
cpolymerase
Primers c(correct canswers)RNA cpolymerase
Feedback
PCR cmakes ca cDNA ccopy, cso cDNA cpolymerase cis cused.
Assuming cthere cis cone ccopy cof cthe ctarget cDNA csequence cbefore cPCR, chow cmany ccopies cof cDNA
care cthere cafter c5 cPCR ccycles?
256
6
65,536
16
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Constantina. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $13.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
80467 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now