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WGU C785 BIOCHEMISTRY FINAL EXAM 2022/2023 REVIEWED (LATEST UPDATE)/GRADED A+
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WGU C785 BIOCHEMISTRY FINAL EXAM 2022/2023 REVIEWED (LATEST UPDATE)/GRADED A+
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WGU C785 BIOCHEMISTRY FINAL EXAM 2022/2023REVIEWED (LATEST UPDATE)/GRADED A+
1. cThe cpolymerase cchain creaction cis ca ctool cused cto cstudy cprotein cstructure.
True
False c(correct canswers)False
Feedback
PCR cis ca ctool cused cto camplify ca cspecific csegment cof cDNA.
What ccolor cis cthe cprimer cin cthe cfollowing cdiagram?
Red
cPurpl
e
Blue c(correct canswers)Red
Feedback
Recall cfrom cthe csection con cDNA creplication cthat cDNA cpolymerase cneeds ca cprimer cto cbegin cDNA
csynthesis. cThis crequirement cmeans cthe cprimers cwill cdirect cthe cDNA cpolymerase cto conly
csynthesize ccomplementary cstrands cof cthe ctarget cDNA. c(Note: cIn cDNA creplication, cthe cprimers
care cRNA cprimers, cwhile cPCR cgenerally cuses cDNA cprimers cbecause cthey care cmore cstable.)
dNTPs care cDNA cnucleotides cused cin cPCR.
,True
False c(correct canswers)True
,Feedback
dNTPs cstands cfor cdeoxynucleotide ctriphosphates, cwhich care cthe cnucleotides cused cin cDNA csynthesis.
A cthermocycler cis ca cmachine cused cfor cPCR cthat cvaries cthe ctemperature cof ca csample.
True
False c(correct canswers)True
Feedback
For cPCR, cin ceach ccycle, cthe ctwo cstrands cof cthe cduplex cDNA care cseparated cby cheating, cthen cthe
creaction cmixture cis ccooled cto callow cthe cprimers cto canneal c(or cpair) cto ctheir ccomplementary
csegments con cthe cDNA. cNext, cthe cDNA cpolymerase cdirects cthe csynthesis cof cthe ccomplementary
cstrands. cThe cuse cof ca cheat-stable cDNA cpolymerase celiminates cthe cneed cto cadd cfresh cenzyme
cafter ceach cround cof cheating c(heat cinactivates cmost cenzymes). cHence, cin cthe cpresence cof
csufficient cquantities cof cprimers cand cdNTPs, cPCR cis ccarried cout csimply cby ccycling cthrough cthe
cdifferent ctemperatures cfor cstrand cseparation, cprimer cannealing, cand cDNA csynthesis.
The cthermocycler cis cthe cmachine cthat cis cused cto cvary cthe ctemperature cof cthe csamples.
DNA cpolymerase cis cused cin cDNA creplication cand cin cPCR.
True
False c(correct canswers)True
Feedback
Both cPCR cand cDNA creplication crequire cDNA cpolymerase cto cmake cnew ccopies cof cDNA.
Which cof cthe cfollowing cchanges ccan cNOT cbe cdetected cusing cPCR?
, Differences cin cDNA
csequence cEpigenetic
c changes cDeletions
Insertions c(correct canswers)Epigenetic cchanges
Feedback
Epigenetic cchanges cdo cnot caffect cthe csequence cof cthe cDNA. cPCR cis cused cto clook cat cthe cDNA
csequence.
Which cof cthe cfollowing ccomponents cis cNOT cused cin cPCR?
DNA ctemplate
cDNA
cnucleotides
cRNA
cpolymerase
Primers c(correct canswers)RNA cpolymerase
Feedback
PCR cmakes ca cDNA ccopy, cso cDNA cpolymerase cis cused.
Assuming cthere cis cone ccopy cof cthe ctarget cDNA csequence cbefore cPCR, chow cmany ccopies cof cDNA
care cthere cafter c5 cPCR ccycles?
256
6
65,536
16
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