Other
HW BIOL 1500 LAB
- Course
- BIOL 1500
- Institution
- Nova Southeastern University
HW BIOL 1500 LAB questions and answers
[Show more]
Seller
Follow
Content preview
HW Lab Questions1. What was the enzyme that was used and why?
The enzyme used was Alkaline phosphatase (ALP) and it was used to because it catalyzes the
hydrolysis of a phosphate group attached to proteins or lipids in the substrate.
2. What created the color change and how?
The hydrolysis the phosphate yields in the substrate para nitrophenol-phosphate (pNPP).
3. Why was the alkaline buffer used for Parts 2 and 3 but not for Part 1?
It was used in parts 2 and 3 to make sure that the pH doesn’t change/
4. Why was it essential to wait to add the enzyme?
It was essential because once you add the enzyme the reaction will start. So if you added it and
let it sit the reaction would've been started and you would be able to see the change in
concentration or absorbance.
5. Collect four peer-reviewed sources on the topic of enzyme properties, the effect of
environmental conditions on enzyme activity, or ALP for the enzyme lab report. Your
response to this question must consist of four appropriate APA-style citations.
Zhang H, Gong L, Liu X, Shao K, Li XZ, Li RX. [Soil Enzyme Activity in Picea schrenkiana
and Its Relationship with Environmental Factors in the Tianshan Mountains, Xinjiang]. Huan
Jing Ke Xue. 2021 Jan 8;42(1):403-410. Chinese. doi: 10.13227/j.hjkx.202004199. PMID:
33372493.
Nakanishi H, Onose S, Kitahara E, Chumchuen S, Takasaki M, Konishi H, Kanekatsu R. Effect
of environmental conditions on the α-glucosidase inhibitory activity of mulberry leaves. Biosci
Biotechnol Biochem. 2011;75(12):2293-6. doi: 10.1271/bbb.110407. Epub 2011 Dec 7. PMID:
22146716.
Zheng YH, Liu J, Ma YZ, Xu Y, Xu F, Hua TC. Temperature effects on enzyme activity of
chicken liver esterase used in calorimetric biosensor. Artif Cells Blood Substit Immobil
Biotechnol. 2012 Feb;40(1-2):125-31. doi: 10.3109/10731199.2011.611470. Epub 2011 Sep 26.
PMID: 21942624.
Phipps J, Chen H, Donovan C, Dominguez D, Morgan S, Weidman B, Fan C, Beyzavi H.
Catalytic Activity, Stability, and Loading Trends of Alcohol Dehydrogenase Enzyme
Encapsulated in a Metal-Organic Framework. ACS Appl Mater Interfaces. 2020 Jun
10;12(23):26084-26094. doi: 10.1021/acsami.0c06964. Epub 2020 Jun 1. PMID: 32478509;
PMCID: PMC7815252.
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller j1377. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $3.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
72042 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now