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Biology 116 Final Exam Questions and Answers

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Biology 116 Final Exam Questions and Answers Determination of what DNA is made of - Ans:-Hershey and Chase marked Virus's with two different radioactive molecules (32p labels DNA, 35s labels protein) 32p was found only inside of infected cells 35s was found in ghost shells Nucleic Acid - Ans:...

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  • October 23, 2024
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Biology 116 Final Exam Questions and
Answers


Determination of what DNA is made of - Ans:✔✔-Hershey and Chase marked Virus's with two different

radioactive molecules (32p labels DNA, 35s labels protein)


32p was found only inside of infected cells


35s was found in ghost shells


Nucleic Acid - Ans:✔✔-polymer of nucleotide monomer


Nucleotide - Ans:✔✔-phosphate group+ sugar+ base


synthesized from nucleoside triphosphates (NTPs)


Nucleoside - Ans:✔✔-sugar + base


Ribose - Ans:✔✔-found in RNA


contains additional O on 2' end


Deoxyribose - Ans:✔✔-found in DNA


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no O on 2' end


Purines - Ans:✔✔-Guanine and Adenine (double ring)


Pyrimidines - Ans:✔✔-Cytosine Uracil and Thymine (single ring)


Polymerization of of Nucleotides - Ans:✔✔-3' hydroxyl group in the sugar links with the phosphate group

of another nucleotide


Dehydration reaction


Polarity of Nucleic acids - Ans:✔✔-has polarity extending from free 5' to 3' end


Watson and Crick model of DNA secondary structure - Ans:✔✔-has exterior sugar-phosphate backbone

with bases facing each other in the interior


Bases of two strands bond Via H bonds


There are hydrophobic interactions between bases of adjacent nucleotides


Strands are antiparallel


Base Bonds - Ans:✔✔-G-C triple bond


A-T double bond


Three Hypothesis for DNA replication - Ans:✔✔-semiconservative replication (right)

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conservative replication


dispersive replication


DNA replication - Ans:✔✔-DNA polymerase Catalyzes DNA synthesis


DNA polymerase requires an OH group to bind to


DNA grows on the 3' end


Origin of replication is where the replication starts


Usually more A-T at origin


dNTP deoxyribonucleoside triphosphate - Ans:✔✔-precursor for DNucleotides


Types of DNA polymerase - Ans:✔✔-DNA polymerase 1 and 3 complete DNA replication


DNA polymerase 2 repairs DNA


Helicase - Ans:✔✔-Breaks H bonds between DNA strands


SSBPs (single strand DNA binding proteins) - Ans:✔✔-stabalizes single strands of DNA after bonds are

broken


Keeps the strands seperate


Topoisomerase - Ans:✔✔-cuts and rejoins DNA downstream to relieve tension force
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