Direct testing - Answer-uses known Ab's to find UNknown Ag's
Indirect testing - Answer-uses known Ag's to find UNknown Ag's
Antisera contains - Answer-Known ab's against specific antigen
What is the most specific technique of producing antisera? - Answer-The monoclonal technique
(animal t...
NUR604 Exam 1 & 2 Review Questions
and Answers
Direct testing - Answer-uses known Ab's to find UNknown Ag's
Indirect testing - Answer-uses known Ag's to find UNknown Ag's
Antisera contains - Answer-Known ab's against specific antigen
What is the most specific technique of producing antisera? - Answer-The monoclonal
technique
(animal technique leads to lots of false +'s because a bunch of extra stuff is in there)
RPR can be seen using___ - Answer-your NAKED eye, aggulation
VDRL can be seen using_____ - Answer-microscope, aggulation
What does a tube in a POSITIVE complement fixation test look like? - Answer--NO
hemolysis has occured so the tube is clear
-means that the ab was present to bind the complement and PREVENT hemolysis
What does a tube look like in a NEGATIVE complement fixation test? - Answer--
hemolysis HAS occured and tube is colored
-Ab was NOT present and therefore the complement was not bound and could cause
hemolysis
What is a titer? - Answer-highest dilution where color is still present
Darker color in an EIA indicates_____ - Answer-more ag-ab complex formed
RIA is _________ even in very low concentrations - Answer-EXTREMELY sensitive
Where is RIA used? - Answer-pregnancy tests, to tell the level of bHCG
Is IFA/ DFA more sensitive? - Answer--IFA (using known antigen to find unknown Ab) is
MORE sensitive, bcs there is less chance of cross rxns when looking for Ab directly!
What is the most sensitive automated method available? Is it direct or indirect? -
Answer-ELISA/EIA
It is indirect testing (using known antigen to find unknown Ab)
Western blot is more ______ than ELISA - Answer-Specific (decreased false +)
PCR is _____________. It uses ___________. - Answer-highly sensitive and specific
, ID of viral/ bacterial DNA
How do you determine if a infection is acute/ chronic? - Answer--antibody titer
-compare IgM and IgG
-IgM elevated in acute
-IgG elevated in chronic
What rises first in regards to testing for HcG in pregnancy? blood or urine? - Answer-
Blood rises first (serum)- Quanitative
Urine- 2nd- qualitative
Monospot rapid slide test is looking for ______ antibodies. What disease is it used for? -
Answer-heterophile ab's
-can be false negatives, esp. early on in disease
-infectious Mono
What microorganism are you testing for w/ syphillis? - Answer-Treponema Pallidum
What is the order of testing for syphillis/ what are the names of the tests? - Answer-1st-
screening test that is highly sensisitive = non-treponemal tests= RPR/ VDRL
2nd- highly specific test that will confirm positive screening test
-FTA-ABS
-MHA-TP
What is immunohematology/ what is it used for? - Answer--id of components in blood
that can cause reactions
-done so that blood transfusions can be given to patients
Agglutinogen vs. Agglutinin - Answer--Agglutinogen- angtigen on surface of RBC
-Agglutinin- antigen in the serum
What are the ways that blood is typed- routine? - Answer--ABO
-RH +/-
What is the universal blood donor? - Answer-O-
What is the universal blood recipient? - Answer-AB+
What is the Rh antibody called? - Answer-D
If you are blood type - you can revieve________blood. - Answer-ONLY - blood! (+ blood
has Ab's against - blood but - blood does NOT have ab's against + blood)
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