Key Concepts in DNA Sequencing and Translation Exam Questions and Answers Already Passed
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Key Concepts in DNA Sequencing and Translation
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Key Concepts In DNA Sequencing And Translation
Key Concepts in DNA Sequencing and Translation Exam Questions and Answers Already Passed
Denaturation - Answers Separates DNA strands at 95°C.
Annealing - Answers Primers bind to complementary sequences on the template DNA at 50-65°C.
Extension - Answers DNA polymerase synthesizes new DNA str...
Key Concepts in DNA Sequencing and Translation Exam Questions and Answers Already Passed
Denaturation - Answers Separates DNA strands at 95°C.
Annealing - Answers Primers bind to complementary sequences on the template DNA at 50-65°C.
Extension - Answers DNA polymerase synthesizes new DNA strands by adding nucleotides at 72°C.
Dideoxynucleotides (ddNTPs) - Answers Terminate DNA strand elongation, resulting in fragments of
various lengths.
Amplicon - Answers The amplified product is the segment of DNA between the two primers.
Agarose Gel Electrophoresis - Answers Technique used to separate DNA fragments by size.
Restriction Map - Answers Created by analyzing band patterns after digesting plasmid DNA with
restriction enzymes.
DNA Quantity Importance - Answers Using a set mass of DNA ensures accurate results; volume =
mass/concentration.
Restriction Enzymes - Answers Bind to specific palindromic sequences in DNA and cut at defined
locations.
Eukaryotic RNA Processing - Answers Involves capping, polyadenylation, and splicing to produce mature
mRNA.
Introns - Answers Non-coding regions removed from pre-mRNA.
Exons - Answers Coding regions that remain in mature mRNA.
Spliceosome - Answers Facilitates mRNA splicing and is composed of snRNPs and proteins.
Alternative Splicing - Answers Allows a single gene to produce multiple protein isoforms.
Processing Prior to Translation - Answers Ensures mRNA stability, correct modifications, and efficient
translation initiation.
Genetic Code - Answers Determined through experiments correlating codon sequences with specific
amino acids.
Wobble Hypothesis - Answers The third nucleotide in a codon can form non-standard base pairs,
allowing flexibility.
Types of RNAs for Translation - Answers mRNA (messenger RNA), tRNA (transfer RNA), rRNA (ribosomal
RNA).
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