Key Concepts in DNA Sequencing and Translation Exam Questions and Answers Already Passed
3 views 0 purchase
Course
Key Concepts in DNA Sequencing and Translation
Institution
Key Concepts In DNA Sequencing And Translation
Key Concepts in DNA Sequencing and Translation Exam Questions and Answers Already Passed
Denaturation - Answers Separates DNA strands at 95°C.
Annealing - Answers Primers bind to complementary sequences on the template DNA at 50-65°C.
Extension - Answers DNA polymerase synthesizes new DNA str...
Key Concepts in DNA Sequencing and Translation Exam Questions and Answers Already Passed
Denaturation - Answers Separates DNA strands at 95°C.
Annealing - Answers Primers bind to complementary sequences on the template DNA at 50-65°C.
Extension - Answers DNA polymerase synthesizes new DNA strands by adding nucleotides at 72°C.
Dideoxynucleotides (ddNTPs) - Answers Terminate DNA strand elongation, resulting in fragments of
various lengths.
Amplicon - Answers The amplified product is the segment of DNA between the two primers.
Agarose Gel Electrophoresis - Answers Technique used to separate DNA fragments by size.
Restriction Map - Answers Created by analyzing band patterns after digesting plasmid DNA with
restriction enzymes.
DNA Quantity Importance - Answers Using a set mass of DNA ensures accurate results; volume =
mass/concentration.
Restriction Enzymes - Answers Bind to specific palindromic sequences in DNA and cut at defined
locations.
Eukaryotic RNA Processing - Answers Involves capping, polyadenylation, and splicing to produce mature
mRNA.
Introns - Answers Non-coding regions removed from pre-mRNA.
Exons - Answers Coding regions that remain in mature mRNA.
Spliceosome - Answers Facilitates mRNA splicing and is composed of snRNPs and proteins.
Alternative Splicing - Answers Allows a single gene to produce multiple protein isoforms.
Processing Prior to Translation - Answers Ensures mRNA stability, correct modifications, and efficient
translation initiation.
Genetic Code - Answers Determined through experiments correlating codon sequences with specific
amino acids.
Wobble Hypothesis - Answers The third nucleotide in a codon can form non-standard base pairs,
allowing flexibility.
Types of RNAs for Translation - Answers mRNA (messenger RNA), tRNA (transfer RNA), rRNA (ribosomal
RNA).
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller TutorJosh. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $7.99. You're not tied to anything after your purchase.
